一株大鳞大麻哈鱼源鲑肾杆菌的检测与鉴定

doi:10.16431/j.cnki.1671-7236.2015.06.040

Abstract

Abstract: A chronic and infective disease occurred on the farmed Chinook salmon (Oncorhynchus tshaivytscha) in a farm in Huairou district of Beijing and it caused high accumulative mortality.The typical clinical symptoms indicated that it might be bacterial kidney disease (BKD).The purpose of this study was to confirm its pathogen.The typical clinical symptoms of sick fish,morphology observation of bacteria in kidney tissue smears and sequence analysis of bacteria DNA were conducted.A number of gram positive brevi bacteria were observed in kidney tissue print.16S rDNA sequence of K1 strain was more than 99% homology with that of Renibacterium salmoninarum and they were in the same cluster.383 and 320 bp fragment were amplified by PCR and nested PCR.The fragments were sequenced and analyzed.The results showed the fragment was shared 100% nucleotide identity with P57 surface protein gene of Renibacterium salmoninarum. It was indicated that these diseased fish were suffering from BKD caused by Renibacterium salmoninarum.This was the first report about Renibacterium salmoninarum detected in farmed fish in China.

Key words: Renibacterium salmoninarum; bacterial kidney disease (BKD); Chinook salmon (Oncorhynchus tshawytscha)

CLC Number:

S852.61

XU Li-pu, WANG Jing-bo, ZHANG Wen, WANG Xiao-liang, CAO Huan, WANG Shu, PAN Yong, JIANG Yu-lin. Detection and Identification of a Strain of Renibacterium salmoninarum Sourced from Cultured Chinook Salmon (Oncorhynchus tshawytscha)[J]. , 2015, 42(6): 1592-1596.

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Detection and Identification of a Strain of Renibacterium salmoninarum Sourced from Cultured Chinook Salmon (Oncorhynchus tshawytscha)

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