梅花鹿鹿茸总蛋白提取方法对双向电泳图谱的影响

›› 2012, Vol. 39 ›› Issue (7): 25-28.

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Methods of Antler Total Protein Extraction on the Effect of Map Quality of Two-dimensional Electrophoresis in Sika Deer

ZHAO Dong^1,2, XING Xiu-mei², YANG Fu-he²

1. School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018,China;
2. State Key Laboratory of Special Economic Animal Molecular Biology,Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction,Ministry of Agriculture,Jilin Province Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences,Jilin 132109,China

Received:2011-12-19 Online:2012-07-20 Published:2012-07-16

Abstract

Abstract: Deer antlers are the only mammalian organs capable of repeated regeneration. To prepare total protein samples of sika deer antler for 2-DE by different extraction methods, and then find a suitable one from the spots number and resolution condition in the map. Total protein extraction kit, manual lysate and acetone precipitation were used and three kinds of 2-DE maps were analyzed. The results showed that spots number and resolution of kit and manual lysate were better than that of acetone precipitation, and stripes in the map of acetone precipitation were visible.

Key words: deer antler; protein sample; extraction methods; two-dimensional electrophoresis

CLC Number:

ZHAO Dong, XING Xiu-mei, YANG Fu-he. Methods of Antler Total Protein Extraction on the Effect of Map Quality of Two-dimensional Electrophoresis in Sika Deer[J]. , 2012, 39(7): 25-28.

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Methods of Antler Total Protein Extraction on the Effect of Map Quality of Two-dimensional Electrophoresis in Sika Deer

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