塔里木马鹿鹿茸VEGF基因克隆及其在不同鹿茸组织中的表达研究

doi:10.16431/j.cnki.1671-7236.2017.05.005

Abstract

Abstract:

This study was aimed to clone the coding sequence of vascular endothelial growth factor (VEGF) from the antler tissue at the top of the Tarim Red deer, and analyze the molecular properties and expression in antler tissue. This study extracted total RNA in antler tip by improved Trizol method and got the VEGF gene by RT-PCR method, then purified and connected it to pMD18-T vector, and conversed it into Escherichia coli DH5α, the expression of the VEGF protein at the top of antler tissue, between the cortex and chopped level of mesenchymal and cartilage layer in Red deer were determined using immunohistochemical method. The results showed that the open reading frame (ORF) length of VEGF gene was 648 bp, and encoding 216 amino acids, sequence homology of the nucleotide were 98.75%, 96.55%, 97.58% and 97.56% comparing with human, cattle, sheep and pig, respectively. The VEGF of Tarim Red deer antler was high similarity with that of human through its sequence analysis and comparison. The VEGF protein were expressed in the top of antler tissue, including skin mesenchymal and cartilage layer from immunohistochemical experiment, but there was no obvious difference. It was predicted that the Tarim Red deer antler might be ideal model of the blood vessel related of regenerative medicine diseases for human research.

Key words: Tarim Red deer antler; vascular endothelial growth factor; cloning; immunohistochemical

CLC Number:

Q786

LIU Jun-feng, WANG Xiao-ting, ZHONG Ting, GUO Xue-feng. Clone and Expression of VEGF Gene in Different Tissue of Tarim Red Deer Antler[J]. , 2017, 44(5): 1289-1294.

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Clone and Expression of VEGF Gene in Different Tissue of Tarim Red Deer Antler

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