Abstract: There were some chickens troubled with a disease which caused the chickens neurosis, declining of egg laying, increasing of egg abnormal, embarrassment as well as diarrhea and so on. The chicken farm was visited and the infected chickens were acquisited. The disease was identified to be the Newcastle disease and produced Escherichia coli afterwards to send infection according to the results of clinical symptom, pathoanatomical examination, bacteriology, orrhology as well as virological examination. Then, real-time fluorescent PCR was done to identify the strain typing of Newcastle disease virus, the result showed that the Newcastle disease virus was midst-velogenic strain.

Key words: Newcastle disease; diagnosis; NDV strains; real-time fluorescent PCR; Escherichia coli