猪星状病毒的分离鉴定及其致病性研究

Abstract

Abstract: Porcine astrovirus (PAstV) infection in pigs could cause diarrhea, vomiting, dehydration and growth retardation and other symptoms, particularly for serious violations to piglets before weaning, and cause some economic losses on the pig industry. In this study, PK-15 cells were used to isolate the epidemic strains of porcine astroviruses in Guangxi, and the virus were identified by using RT-nPCR, indirect immunofluorescence, electron microscopy, Western blotting analysis and the experiment of pathogenicity. These could provide the basis to further study the molecular biology, pathogenesis and animal models of porcine astrovirus.

Key words: porcine astrovirus; isolation; identification; animal experiments

CLC Number:

S855.3

LAN Jia-nuan, GUO Xuan, LIU Lei, ZHANG Min-xiu, LU Bing-xia, LIAO Cheng-qiu, HUANG Fu-biao, HUANG Wei-jian. Isolation, Identification and Pathogenicity of Porcine Astroviruses[J]. , 2012, 39(7): 199-203.

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Isolation, Identification and Pathogenicity of Porcine Astroviruses

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