›› 2012, Vol. 39 ›› Issue (6): 72-75.

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Development of a Loop-mediated Isothermal Amplification Method for Rapid Detection of Capripoxvirus

HUANG He1,2, LI Ying-guo2, XIAO Jin-wen2, NIE Fu-ping2, WANG Yu2, LIU Li1   

  1. 1. College of Animal Science and Technology,Southwest China University,Chongqing 400715,China;
    2. Chongqing Import and Export Food Safety Engineering Center,Chongqing 400020,China
  • Received:2011-12-04 Online:2012-06-20 Published:2012-07-02

Abstract: A method for detection of capripoxvirus (CaPV) through the loop-mediated isothermal amplification (LAMP) was established. The conditions for amplification of CaPV DNAs were optimized using LAMP Real Time Turbidimeter LA-320. It was found that at 62 ℃ for 60 min, highly efficient amplification of the goat pox virus(GPV), sheep pox virus(SPV)and bovine lump skin disease virus (LSDV) were achieved. The minimum detection of viral nucleic acid content was up to 3.1×10-2 pg/μL, 104 times higher than the PCR method recommended by OIE. The method was rapid, specific and easy to operate.

Key words: CaPV; GPV; SPV; LSDV; LAMP

CLC Number: