鹅细小病毒侵染雏鹅组织器官的氧化/脱氢酶及同工酶研究

doi:10.16431/j.cnki.1671-7236.2015.08.023

Abstract

Abstract: The enzyme was efficient active gene product of catalyzing metabolic pathway.Stress environment and genetic basis led the differences of isozyme structure or activity, which specific changes of the kinds and activities of enzyme were showed in different tissues and developmental stages.Here, lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), peroxydase (POD) and catalase (CAT) were respectively extracted from brain, heart, liver, lung and spleen tissues and organs of goslings infected by goose parvovirus (GPV) and control group.The results of PAGE and biochemical analysis showed that there respectively were 3 and 1 slow-zone CAT isozyme bands deletion in liver and spleen of goslings infected by GPV.There respectively were 1 new enzyme band of POD isozyme in the brain, heart and spleen of goslings infected by GPV, but 1 enzyme band of POD isozyme deletion in the lung of goslings infected by GPV.It only showed 1 to 2 slow-zone LDH and ADH isozyme bands in organ and tissue of goslings infected by GPV, and deleted 1 slow-zone ADH isozyme band in the lung of goslings infected by GPV.The activity of ADH from goslings infected by GPV were reduced 13.61% to 61.08%, but increased 1.2 times in the heart of goslings infected by GPV.The activity of POD, CAT and LDH from goslings infected by GPV were higher 1.2 to 6, 1.5 to 3.5, 2 to 2.5 times than that of control group, respectively.The activity of CAT reduced 40.29% and 94.68% in the brain and lung of goslings infected by GPV.The activity of LDH reduced 75.93% and 91.81% in liver and lung of goslings infected by GPV.Those results indicated that the interaction of GPV stress and host oxidase/dehydrogenase enzyme gene expression resulted in the biochemical characteristics variation of activity and isozyme patterns of oxidase/dehydrogenase enzyme, and directly or indirectly affected metabolic approach and physiological pathological function on goslings infected by GPV.Therefore, sensitive oxidase/dehydrogenase was the effective marker of pathological mechanism on investigation of virus genotype interaction with genetic susceptibility of the host.

Key words: goslings; goose parvovirus (GPV); dehydrogenase; oxidase; isozyme

CLC Number:

S852.33

ZHU Xin-chan, ZHANG Zhao-bin, HUANG Yun-ge, YANG Li-jin. Study on the Oxidase/Dehydrogenase Enzymes and Isozyme from Tissues and Organs of Goslings Infected by Goose Parvovirus[J]. , 2015, 42(8): 2081-2090.

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Study on the Oxidase/Dehydrogenase Enzymes and Isozyme from Tissues and Organs of Goslings Infected by Goose Parvovirus

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