猪繁殖与呼吸综合征病毒主要结构蛋白基因半定量 RT-PCR方法的建立及其在RNA干扰研究中的应用

Abstract

Abstract: Three pairs of primers were designed according to the sequence of PRRSV VR2332 strain to amplify GP5、M and N protein gene respectively, a semi-quantitative RT-PCR method was developed. Different shRNA expressing vectors were cotransfected into HEK293A cells with vectors expressing structural proteins of PRRSV, and the mRNA levels of target gene were assayed by semi-quantitative RT-PCR. The results showed that the mRNA levels of structural proteins were inhibited from 36% to 69%, and pSi-N3 and pSi-G1 showed the strongest inhibition effect. It indicated that the method was suitable for relative quantitation of the main structural proteins of PRRSV.

Key words: PRRSV; structural protein; semi-quantitative RT-PCR; RNA interference

CLC Number:

S854.4⁺3

HUANG Juan, SHAN Hu. Development and Application of Semi-quantitive RT-PCR for the Main Structural Protein Genes of Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2012, 39(6): 22-26.

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Development and Application of Semi-quantitive RT-PCR for the Main Structural Protein Genes of Porcine Reproductive and Respiratory Syndrome Virus

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