›› 2012, Vol. 39 ›› Issue (5): 27-30.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequencing Analysis M1 Gene of Avian Influenza Virus Strain A/duck/Fujian/31/2007(H5N1)

BU Ri-e1,2, REN Xiao-feng3, LI Ming-gang4, WU Jin-hua1,2, SUN Li-jie1,2   

  1. 1. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China;2. Research Institute of Pathogenic in Milk, Inner Mongolia University for Nationalities, Tongliao 028043, China;3. Collgege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;4. Beijing Zhuang Di Hao He Biomedical Technology Co., Ltd., Beijing, 100043, China
  • Received:2011-11-21 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20

Abstract: A pair of primer was designed according to the sequences of avian influenza virus (AIV) on GenBank website. Total RNA from AIV was taken as template to make RT-PCR amplification. The M1 gene of AIV was amplified by RT-PCR.M1 gene was 759 bp in length, encoding 252 amino acids. Comparing with other published M1 cDNA sequences. It showed that the homology of M1 gene was from 99.1% to 99.6%, and the homology of amino acids was from 98.8% to 99.60%.Bioinformatics analysis of this sequence showed that the protein of M1 was hydrophilicand highly antigenic. M1 includes six potential N-glycosylation sites, three potential proteinkinase C phosphorylation sites, two potential caseinkinaseⅡ phosphorylation sites.

Key words: avian influenza virus; M1 gene; cloning; sequence analysis

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