犬瘟热病毒疫苗变异株的分离及其P1蛋白的表达、鉴定

›› 2012, Vol. 39 ›› Issue (5): 30-34.

• 生物技术 • Previous Articles Next Articles

Isolation of Canine Distemper Virus Vaccine Variant Strain and the Expression and Identification of P1 Recombinant Protein in E.coli

YU Ping-ping^1,2, JIA Hong², HOU Shao-hua², YUAN Wei-feng², GUO Xiao-yu², CUI Zhi-zhong¹, ZHU Hong-fei²

1. Animal Science and Technology Institute, Shandong Agricultural University, Taian 271017, China;2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:1900-01-01 Revised:2011-11-10 Online:2012-05-20 Published:2012-05-20

Abstract

Abstract: A canine distemper virus was isolated from an infected dog in a pet hospital in Hebei. It had 99.5% homology with vaccine strain by sequencing of H gene, and three amino acids changed that had no effect on glycosylation sites. The P1 gene of this strain was cloned into pET32a(+) vector. The recombinant plasmid was transformed into BL21(DE3),Rosetta and protein expression was induced by IPTG. The expression protein had a molecular weight of 50 ku. And expressed mainly in form of dissolubility body. The expressed protein reacted specially with CDV serum in Western blotting and ELISA. IFA result showed that the P1 protein fixed in the cell nucleus.

Key words: canine distemper virus; variant strain; P1 recombinant protein; antigencity

CLC Number:

YU Ping-ping;JIA Hong;HOU Shao-hua;YUAN Wei-feng;GUO Xiao-yu;CUI Zhi-zhong;ZHU Hong-fei. Isolation of Canine Distemper Virus Vaccine Variant Strain and the Expression and Identification of P1 Recombinant Protein in E.coli[J]. , 2012, 39(5): 30-34.

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Isolation of Canine Distemper Virus Vaccine Variant Strain and the Expression and Identification of P1 Recombinant Protein in E.coli

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