蜥蜴利什曼原虫在小鼠体内的分布及增殖研究

›› 2012, Vol. 39 ›› Issue (2): 48-51.

• 生物技术 • Previous Articles Next Articles

Distribution and Proliferation of Leishmania tarentolae in BALB/c Mice

LI Lin^1,2, MA Li-min^1,2, REN Xiu-mei¹, HE Ye¹, LI Ye¹, SHANG Li-min², HU Le-peng², XUE Hui-liang², LI Dan², JIN Zhao¹, XIA Zhi-ping²

1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Military Veterinary Institute,Academy of Military Medical Sciences of PLA,Changchun 130122,China

Received:2011-11-11 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

Abstract

Abstract: BALB/c mice inoculated with Leishmania tarentolae parasites which express fluorescent protein by intraperitoneal routes. Frozen sections of their organs were stained with fluorescent dye and observed by fluorescence microscopy. The results showed that Leishmania tarentolae were observed in heart,liver,spleen,lung and kidney of BALB/c mice. In addition,Leishmania real-time quantitative PCR method was established successfully and used to detect Leishmania tarentolae in BALB/c mice. These results indicated that the proliferation curve of Leishmania tarentolae in BALB/c mice was wavy in 13 days. The results for the study of Leishmania tarentolae will provide the theoretical basis for pathogenesis,vaccine development in humans and animals.

Key words: Leishmania tarentolae; fluorescent staining; real-time quantitative PCR

CLC Number:

S879.1

LI Lin;MA Li-min;REN Xiu-mei;HE Ye;LI Ye;SHANG Li-min;HU Le-peng;XUE Hui-liang;LI Dan;JIN Zhao;XIA Zhi-ping. Distribution and Proliferation of Leishmania tarentolae in BALB/c Mice[J]. , 2012, 39(2): 48-51.

[1]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[2]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[3]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[4]	LI Heng, ZI Xiangdong. Cloning and Expression in Cumulus-oocyte Complexes of Yak TEDDM1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1284-1293.
[5]	CHEN Lei, YUAN Feng, HE Sangang, MAYILA, LI Wenrong. Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 425-432.
[6]	DAI Zhenglie, ZHU Jingjing, CHEN Zhenyu, ZHOU Xiaolong, WANG Han, LI Xiangchen, ZHAO Ayong, YANG Songbai. Cloning, Bioinformatics Analysis and Expression of Porcine SENP1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4382-4390.
[7]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[8]	LI Zhiqiang, TAO Chenyu, WEI Aolong, JIA Qing. Establish on Real-time Quantitative PCR Method for Purity Detection of Porcine Sex-controlled Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3682-3690.
[9]	WANG Zhengfan, ZHU Lisai, WANG Juan, LI Yiyun, XIANG Rui, YING Biyun, WANG Guiping, JIA Aiqing, BAI Aiquan. Establishment and Preliminary Application of Duplex Real-time Quantitative PCR for Detection of PDCoV and TGEV [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3855-3863.
[10]	LI Anqi, JIANG Lei, SU Xiaotong, ZAN Linsen, WANG Hongbao. Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2359-2367.
[11]	LI Jinghui, XING Siyuan, WANG Xicai, LI Qinghe, ZHAO Guiping, ZHANG Yonghong, WEN Jie, LIU Ranran. Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1828-1836.
[12]	ZHANG Ji, YI Ming, CHENG Chaoyou, CHENG Junhua, FU Zhengxian, YANG Dewen, WU Xue, QIU Ganyuan, LIU Ruoyu. Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep [J]. , 2019, 46(7): 1899-1906.
[13]	HUA Min, HE Xinwei, WAN Run, CHENG Zhentao, ZHOU Bijun, WEN Ming. Sequence Analysis of Cofilin2 Gene and Its Expression in Different Tissues of Sansui Ducks [J]. , 2018, 45(4): 858-865.
[14]	WANG Shu-juan, LIU Mei-fen, YAN Ruo-qian, BAN Fu-guo, ZHAO Xue-li, MA Zhen-yuan, WANG Hua-jun, WANG Cui, ZHAO Ming-jun. Establishment and Application of Real-time Quantitative PCR Assay for Detection of Classical Swine Fever Virus [J]. , 2017, 44(7): 2112-2118.
[15]	TANG Lian-fei, YU Si-yu, ZHOU Yu. Establishment of Digital RT-PCR Assay for Detection of Swine Influenza Virus H3N2 Subtype [J]. , 2017, 44(6): 1847-1853.

Distribution and Proliferation of Leishmania tarentolae in BALB/c Mice

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments