野生乌苏里貉18SrDNA全序列的克隆测序及系统进化树分析

Abstract

Abstract: In this study, 18S rDNA gene was extracted from wild Nyctereutes procyonoides matschie's meat of ear, and amplified by polymerase chain reaction (PCR) method, then cloned and sequenced. A lengh of 1831 bp was cloned, the sequences had been sent to GenBank and registered, comparison of the sequence with that of other animals 18S rDNA gene was carried out through BLAST. The results showed that the homologies of the nucleotide sequence were above 90% with Nyctereutes procyonoides matschie, and 18S rDNA gene was conserved in evolution, which could be used as phylogenetic analysis of the important reference.

Key words: wild Nyctereutes procyonoides matschie; cloning and sequencing; 18S rDNA; phylogenetic

CLC Number:

Q813

LIU Cheng-gang;DU Zhi-heng;BAI Xiu-juan. Cloning,Sequencing and Phylogenetic Analysis 18S rDNA Gene in Wide Nyctereutes Procyonoides Matschie[J]. , 2012, 39(2): 117-120.

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Cloning,Sequencing and Phylogenetic Analysis 18S rDNA Gene in Wide Nyctereutes Procyonoides Matschie

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