›› 2012, Vol. 39 ›› Issue (12): 177-179.

Previous Articles     Next Articles

Isolation and Identification of a New Type of Duck Hepatitis Virus by RT-PCR

MA Chao-ying   

  1. Veterinary Station of Haixi Tongpu Town, Ulan County, Qinghai Province, Ulan 817100, China
  • Received:2012-04-24 Online:2012-12-20 Published:2012-12-19

Abstract: In order to investigate the pathogenesis of duck field, one strain of duck hepatitis virus was isolated from the duck field, determination of the virus in duck embryo ELD50 for 10-5.53/0.2 mL by Reed-Muench method, animal regression test showed attack ducklings reproduce original disease and clinical symptoms and pathological changes, type DHV-Ⅰ positive serum on strains isolated virus without neutralization, RT-PCR identification the isolated strain of the virus is a new type of duck hepatitis virus.

Key words: duck hepatitis virus; isolation; RT-PCR; identification

CLC Number: