基于线粒体DNA控制区(mtDNA D-loop)序列分析贵妃鸡的遗传多样性

Abstract

Abstract: In this paper, the mitochondrial DNA D-loop sequence(520 bp in length) of 30 individuals from Royal chicken of the first Ⅰ region sequences were analyzed by PCR product direct sequencing technology. The results revealed that the average values of base composition of A,G,C and T in the sequence were 26.8%, 13.0%, 31.1% and 29.1%, respectively. And there were 13 nucleotide polymorphic sites which were transition neither transversion nor insertion/deletion. The haplotype diversity (Hd) and average nucleotide diversity (Pi) of the sites were 0.538 and 0.0059, and the value of Tajima’s D was -0.67065. Using the NJ method showed that the Royal chicken origined in the Red Gallus.

Key words: Royal chickens; mtDNA D-loop; genetic diversity; haplotype

CLC Number:

S816.79

WU Chan, LI Hui, LI Jing-rui, ZHANG Da-quan, XIE Guo-wu, KONG Yan, YI Heng-jie. Genetic Diversity of Royal Chicken Basesd on Mitochondrial DNA D-loop Region Sequence[J]. , 2012, 39(12): 137-140.

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Genetic Diversity of Royal Chicken Basesd on Mitochondrial DNA D-loop Region Sequence

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