影响Fat-1转基因水牛胚胎发育因素的研究

Abstract

Abstract: The effects of injecting timing after fertilization (5 to 6, 9 to 10, 13 to 14, 17 to 18, 21 to 22 h) and exogenous DNA concentrations (10, 50 and 100 μg/mL) on the development of Fat-1 transgenic embryo were investigated. The results showed that the EGFP expressing rate and blastocysts’ EGFP expressing rate of 9 to 10 h group were the highest, the EGFP expressing rate were significantly higher than 5 to 6 h group(P<0.01) and the blastocysts’ EGFP expressing rate significantly higher than 21 to 22 h group(P<0.01);in the concentration test, the EGFP expressing rate and blastocysts’ EGFP expressing rate of 50 μg/mL group were the highest, and significantly higher than 100 μg/mL(P<0.01).

Key words: buffalo; transgenic; Fat-1; embryos

CLC Number:

S813.1

WANG Jian, PANG Chun-ying, DENG Ting-xian, YANG Chun-yan, ZHENG Hai-ying, HUANG Fen-xiang, YANG Bing-zhuang, LIANG Xian-wei. Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo[J]. , 2012, 39(12): 121-124.

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Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo

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