禽流感病毒HA-M2融合表位与EGFP蛋白在鸭胚成纤维细胞中的表达

Abstract

Abstract: In this study, two T cell epitopes genes of hamagglutinin conserved domains of avian influenza virus H9N2 strain and M2 gene of extracellular domain were synthetized and joined as HA-M2 multiple-epitopes fusion gene of avian influenza virus by RT-PCR. The recombinant eukaryotic expression plasmid of HA-M2 fusion gene containing enhanced green fluorescent protein (EGFP) report gene was constructed, and transfected by lipofectin to prepared duck embryo fibroblasts (DEF). The fluorescence expression was directly detected with fluorescence microscope, and the expression of HA-M2 was tested by RT-PCR and indirect immunofluorescence assay (IFA) respectively. The results indicated the successful construction of the eukaryotic expression plasmid HA-M2-pEGFP-N1, and showed that HA-M2 gene was expressed efficiently in transfected DEF. It provided a basis for the study of new universal influenza vaccines and further research on interaction of HA gene and its target cells and as for infection mechanism of AIV.

Key words: avian influenza; epitopes fusion gene; EGFP; duck embryo fibroblasts

CLC Number:

S858.3

WANG Shan-hui, WANG Wen-xiu, SHEN Zhi-qiang, CHI Xian-feng, GAO San-yang. Expression of HA-M2 Multiple-Epitopes Fusion Protein of Avian Influenza Virus and EGFP Protein in Duck Embryo Fibroblasts[J]. , 2012, 39(11): 27-30.

References

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Expression of HA-M2 Multiple-Epitopes Fusion Protein of Avian Influenza Virus and EGFP Protein in Duck Embryo Fibroblasts

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