2株猪繁殖与呼吸综合征病毒全基因序列分析

Abstract

Abstract: Ten gene fragments contains two strains of porcine reproductive and respiratory syndrome virus which were designated ZS-GD strain and ZH-GD strain complete genome were amplified respectively by RT-PCR, and cloned into pMD18-T vector for sequencing. Phylogenetic analysis of two strains of PRRSV complete genome sequence by bioinformatics software showed that the complete genome of ZS-GD and ZH-GD were 15320 bp, the genome of ZS-GD strain and ZH-GD strain shared 88.1% to 97.8% and 88.4% to 98.0% of nucleotide homology with American type strain, respectively; shared 60.6% and 60.4% of nucleotide homology with Europe type strain(Lelystad virus). There were 30aa depletion in Nsp2 protein of two stains. The results showed that these two strains were depletion and variant strain of American type.

Key words: PRRSV; genome; sequencing; analysis

CLC Number:

ZHANG Min-ze, YAN Ming-fei, XIE Jie-xiong, HUANG Zhen, CAO Zong-xi, WU Xin-wei, GUO Si-hu, ZHANG Gui-hong. Sequence Analysis of Complete Genome of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(10): 49-52.

References

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Sequence Analysis of Complete Genome of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus

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