犬瘟热病毒RT-LAMP检测方法的建立及初步应用

›› 2012, Vol. 39 ›› Issue (10): 45-49.

Previous Articles Next Articles

Establishment and Preliminary Application Development of a Loop-mediated Isothermal Amplification Assay for Detection of Canine Distemper Virus

HU Jia-xin^1,2, WEN Yong-jun², HUO Zhi-yun^1,2, SHI Xin-chuan², YANG Bo-chao², WU Hua², GAO Wei-fan¹

1. Shenyang Agricultural University,Shenyang 110161,China;
2. Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130122,China

Received:2012-03-05 Online:2012-10-20 Published:2012-10-19

Abstract

Abstract: This paper developed a new method in rapid detection of canine distemper virus(CDV) based on loop-mediated isothermal amplification(LAMP)assay which overcame the difficulties found in other measures,such as efficiency and high cost. In order to improve the specificity and sensitivity, we designed 6 primers recognizing the 8 conservative sequences on N gene of CDV. The result could be easily confirmed by agarose gel electrophoresis, color reaction or turbidity comparison. In conclusion, The newly developed RT-LAMP detection method for CDV was more specific, sensitive, simple and rapid than the others, even more the proceeding reaction was visible by the naked eye, and that was also to say, it offered a simple solution in field veterinary diagnose for CDV control.

Key words: canine distemper virus(CDV); loop-mediated isothermal amplification(LAMP); N gene

CLC Number:

S852

HU Jia-xin, WEN Yong-jun, HUO Zhi-yun, SHI Xin-chuan, YANG Bo-chao, WU Hua, GAO Wei-fan. Establishment and Preliminary Application Development of a Loop-mediated Isothermal Amplification Assay for Detection of Canine Distemper Virus[J]. , 2012, 39(10): 45-49.

References

1 王宏俊,丁少忠.核酸探针检测犬瘟热病毒方法的建立和初步应用[J].中国兽医杂志,2006,40(5):19~22.
2 刘中勇,曾小娜,朱道中,等.猪瘟病毒RT-LAMP快速检测方法的建立[J].中国畜牧兽医,2010,37(6):71~74.
3 李元迎,李瑞影,郭进.犬瘟热研究进展[J].华南农学报,2009,24:246~250.
4 陈蕾.猪瘟病毒RT-LAMP快速诊断试剂盒的研制与应用.北京:中国兽医药品监察所,2009.
5 An D J, Kim T Y,Song D S, et al. An immunochromatogrphy assay for rapid antermorterm diagnosis of dogs suspected to have canine distemper[J].J Virol Methods, 2008,147(2):244~249.
6 Chen H T, Zhang J, Ma L N, et al. Rapid pre-clinical detection of classical swine fever by reverse transcription loop-mediated isothermal amplification[J].Mol Cell Probes, 2009,23(2):71~74.
7 Chen H T, Zhang J, Ma Y P, et al. Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues[J].Mol Cell Probes, 2010,24(2):104~106.
8 Chen H T, Zhang J, Sun D H, et al. Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus[J].J Virol Methods, 2008a,151(2):200~203.
9 Chen H T, Zhang J, Sun D H, et al. Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus[J].J Virol Methods, 2008b,153(2):266~268.
10 Gabriella E, Nicola D, Vito M, et al. Detection of canine distemper virus in dogs by real-time RT-PCR[J].J Virol Methods, 2006,136:171~176.
11 Kurosaki Y, Takada A, Ebihara H, et al. Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification[J]. J Virol Methods, 2007,141(1):78~83.
12 Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers[J].Mol Cell Probes, 2002,16(3):223~229.
13 Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Res, 2000,28 (2):E63.
14 Parida M, Horioke K, Ishida H, et al. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription loop-mediated isothermal amplification assay[J]. J Clin Microbiol, 2005,43(6):2895~2903.
15 Toriniwa H, Komiya T. Rapid detection and quantification of Japanese encephalitis virus by real-time reverse transcription loop-mediated isothermal amplification[J]. Microbiol Immunol, 2006,50(5):379~387.

[1]	WU Yu-xi, LI Pu-jun, WANG Juan, YANG Fa-long. Loop-mediated Isothermal Amplification Method and Its Application in Pathogen Detection [J]. , 2016, 43(2): 389-393.
[2]	DAN Xiao-ya;XUE Qiang;ZOU Ming-qiang;DONG Ying. The Improvement and Application of Reverse Transcription Loop-mediated Isothermal Amplification for Detection of Pathogenic Microorganisms [J]. , 2012, 39(2): 52-56.

Establishment and Preliminary Application Development of a Loop-mediated Isothermal Amplification Assay for Detection of Canine Distemper Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments