饲喂不同抗生素对肉牛废弃粪便中四环素耐药基因数量及持久性的影响

Abstract

Abstract: This study was investigated the effects of administering beef cattle antimicrobials at subtherapeutic concentrations on the abundance and persistence of tetracycline resistance genes within the whole microbial community of fecal waste.Cattlwere administered chlortetracycline, chlortetracycline plus sulfamethazine and no antimicrobials. Model fecal deposits (n=3) were prepared by mixing fresh feces from pens into a single composite sample. Real-time PCR was used to measure concentrations of tetracycline resistance genes tet(B), tet(C), tet(L), tet(M), tet(W) and 16S rRNA in DNA extracted from composite feces after 7, 14, 28, 42, 56, 70, 84, 98, 112, 126 and 175 days in the field. The concentrations of 16S rRNA in feces were similar across treatments and increased by day 56. Generally, initial concentrations of tetracycline resistance genes were greater (P＜0.05) in fecal pats from animals fed chlortetracycline. For all fecal treatments, tet(B) and tet(C) increased 1 to 2 log units by 56 d, and then decreased to the initial 7 d levels by 175 d (P＞0.05). The concentrations of tet(M) and tet(W) were greater than other tetracycline resistance determinants. Tetracycline resistance genes could persist in fecal waste from cattle beyond 175 days and the initial load of some genes might underestimate concentrations at later time points. Temporal changes in the concentrations of resistance genes were likely due to shifts in microbial populations.

Key words: cattle fecal deposits; tetracycline; antimicrobial resistance gene

CLC Number:

S816.73

JIN Xin, ZHANG Wen-guang, ZHANG Yan-jun, SU Rui, WANG Rui-jun, LI Jin-quan. Abundance and Persistence of Tetracycline Resistance Genes in Fecal Depositsy from Beef Cattle Fed Different Subtherapeutic Antibiotics[J]. , 2012, 39(10): 101-105.

References

1 金鑫,苏蕊,张文广,等. 3种四环素耐药基因型肉牛肠道大肠杆菌对四环素类抗生素的耐药性分析[J].中国畜牧兽医,2012,39(5):187~191.
2 Alexander T W, Reuter T,Sharma R,et al. Longitudinal characterization of resistant Escherichia coli in fecal deposits from cattle fed subtherapeutic levels of antimicrobials. Appl Environ Microbiol,2009,75:7125~7134.
3 Barton M D. Antibiotic use in animal feed and its impact on human health. Nutr Res Rev,2000,13:279~299.
4 Blake D P,Hillman K,Fenlon D R,et al. Transfer of antibiotic resistance between commensal and pathogenic members of the Enterobacteriaceae under ileal conditions. Journal of Applied Microbiology,2003,95:428~436.
5 Chen J,Yu Z,Michel F C,et al. Development and application of real-time PCR assays for quantification of erm genes conferring resistance to macrocodes-lincosamides-streptogramin B in livestock manure and manure management systems. Appl Environ Microbiol, 2007,73(14):4407~4416.
6 Ghosh S, LaPara T M. The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria.The ISME Journal, 2007, 1:191~203.
7 Lay C,Sutren M,Rochet V,et al. Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota. Environ Microbiol,2005,7:933~946.
8 Patterson A J,Colangeli R,Spigaglia P,et al. Distribution of specific tetracycline and erythromycin resistance genes in environmental samples assessed by macroarray detection. Environ Microbiol,2007,9:703~715.
9 Peak N,Knapp C W,Yang R K,et al. Abundance of six tetracycline resistance genes in wastewater lagoons at cattle feedlots with different antibiotic use strategies. Environ Microbiol Letters,2007,9:143~151.
10 Roberts M C. Update on acquired tetracycline resistance genes. FEMS Microbiol Lett,2005,245:195~203.
11 Schmitt H,Stoob K,Hamscher G,et al. Tetracyclines and tetracycline resistance in agricultural soils: Microcosm and field studies. Microbiol Ecol, 2006, 51:267~276.
12 Schuster C J,Ellis A G,Robertson W J,et al. Infectious disease outbreaks related to drinking water in Canada, 1974~2001. Can J Pub Health,Revue Canadienne De Sant Publique, 2005,96(4):254~258.
13 Seville L A,Patterson A J,Scott K P,et al. Distribution of tetracycline and erythromycin resistance genes among human oral and fecal metagenomic DNA. Microb Drug Resist,2009,15:159~166.
14 Shery P G,Chery L W, Josee H,et al. Associations between antimicrobial resistance genes in fecal generic Escherichia coli isolates from cow-calf herds in western Canada. Appl Environ Microbiol,2008,74(12): 3658~3666.
15 Sinton L W,Braithwaite R R,Hall C H,et al. Survival of indicator and pathogenic bacteria in bovine feces on pasture. Appl Environ Microbiol,2007,73:7917~7925.
16 Unc A,Goss M J. Transport of bacteria from manure and protection of water resources. Appl Soil Ecol,2004,25:1~18.

[1]	LIU Baoling, CHU Pinpin, LI Chunling, YANG Dongxia, JIANG Zhiyong, ZHANG Kunli, SONG Shuai, GOU Hongchao, CAI Rujian. Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2796-2804.
[2]	XU Xiangyue, MA Wenjin, AN Boyu, CHENG Guyue, HUANG Lingli. Advances on Risk Assessment of Tetracycline Antibiotics in the Environment [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 948-957.
[3]	WANG Xuejun, TAN Aijuan, LV Shiming, DU Anding, CHEN Boli, ZHANG Shengxun. Detection of Resistance and Resistance Genes of Swine Escherichia coli in Guizhou to Tetracyclines [J]. , 2018, 45(5): 1367-1373.
[4]	WU Kainian, WANG Li, LI Chenyang. Drug Resistance Analysis of Aminoglycosides and Tetracyclines in Aeromonas sobria Isolated from Fish [J]. , 2018, 45(4): 1059-1066.
[5]	WANG Xu-dan, LIU Jing, WANG Geng-nan, ZHANG Dong-xu, LIU Ju-xiang. Residue Detection of Tetracyclines in Eggs by High Performance Liquid Chromatography [J]. , 2016, 43(6): 1579-1584.
[6]	LIU Yuan, LIU Wen-li, CUI Yi-fang, LIU Jian-ye, HUANG Xiao-yong, LIU Xiao-ye, YAN Zhao-yang, CAO Jie, DING Shuang-yang. Study on Optimizations of Oxytetracycline Residues Detection Method in Milk and its Clinical Elimination [J]. , 2016, 43(5): 1335-1342.
[7]	HUANG Wen-ming, OU Yang-yun, ZHANG Min-ze, HUANG Zhen, XIE Jie-xiong, SUN Long, ZHANG Gui-hong. Resistance Characteristics and Distribution of Tetracycline Resistance Genes in Streptococcus suis from Pigs in Guangdong [J]. , 2013, (5): 54-58.
[8]	LI Jia-jia, ZHAO Zhen-sheng, ZHANG Dai-bao, NIE Tian-tian, WANG Zhen-hua. Research Progress on Immunoassay in Tetracycline Residues [J]. , 2013, 40(3): 227-229.
[9]	NIE Wen-ying, TANG Cheng-ming, WANG Jian-xia, LUO Xiao-qin, FENG Cai-wei. Development of Colloidal Gold Rapid Detection Method of β-lactams and Tetracyclines in Milk [J]. , 2013, 40(12): 70-75.
[10]	XIE Yu, WANG Liang, ZHANG Dong-jie, LIU Di, SUN Ya-meng. Construction of Tetracycline-inducible MC4R Gene Eukaryotic Expression Vector in Pig [J]. , 2013, 40(1): 13-16.
[11]	JIN Xin;SU Rui;ZHANG Wen-guang;ZHANG Yan-jun;WANG Rui-jun;LI Jin-quan. Analysis on Antimicrobial Susceptibility to Tetracycline Analogues for Three Genotypes of Tetracycline Resistance in Escherichia coli Isolates from Feedlot Cattle [J]. , 2012, 39(5): 187-191.
[12]	ZHOU Wen-zhong;BI Ke-dong;ZOU Ben-ge;LI Hong;ZOU Ming;ZHANG Qi-di. Studies on the Combined Antibacterial Activity of Florfenicol and Oxytetracycline Hydrochloride in vitro [J]. , 2011, 38(7): 201-203.
[13]	LIANG Hong-bin;LIU Jing-qing;LI Fu-huang;XU Hai-tao;NIU Jia-ying;LI Jing;SUN Yang;GUO Yong;LIU Yun-hai. Comparison of Different Methods for Boar Sperm Capacitation by CTC Staining [J]. , 2010, 37(4): 171-173.
[14]	ZHANG Gui-xian. Synthesis and Identification of Artificial Antigen against Tetracycline Hydrochloride [J]. , 2010, 37(3): 171-174.
[15]	SUN Hong-li; LIU Zhi-qin; XU Hong-xin; YANG Bao-zhu; REN Guo-qiang. Subchronic Toxic Experiment of Oxytetracycline Herb Residues Removed the Titer [J]. , 2010, 37(1): 164-165.

Abundance and Persistence of Tetracycline Resistance Genes in Fecal Depositsy from Beef Cattle Fed Different Subtherapeutic Antibiotics

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments