定量检测牛雌性发育候选基因FOXL2表达方法的建立

›› 2012, Vol. 39 ›› Issue (1): 1-5.

• 生物技术 • Next Articles

Construction of Bovine Sex Related Genes FOXL2 Standard Plasmid and Standard Curve by Real-time RT-PCR

XU Chao^1,2, DU Wei-hua², ZHAO Jia-ping¹, YU Da-wei², WANG Dong², HAO Hai-sheng², LI Guang-yu¹, ZHU Hua-bin²

1. Institute of Special Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Jilin 132109,China;2. Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China

Received:2011-06-20 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20

Abstract

Abstract: The primer was designed based on forkhead transcription factor 2(FOXL2), according to the sequence which was submitted on GenBank. A recombinant plasmid contained cDNA fragment of FOXL2 gene was constructed and used for the standard substance for quantitative detection of mRNA of the FOXL2 gene of Holstein, a Real-time PCR was developed for detection of FOXL2 gene. The results revealed this method had a good specificity, and the sensitivity was up to 10¹, range from 10¹ to 10⁶. The cutoff value Ct had a good linear correlation with PCR samples. This method can detecte FOXL2 gene in bovine quantitatively.

Key words: FOXL2 gene; Real-time quantitative PCR; TaqMan probe; standard curve

CLC Number:

Q786

XU Chao;DU Wei-hua;ZHAO Jia-ping;YU Da-wei;WANG Dong;HAO Hai-sheng;LI Guang-yu;ZHU Hua-bin. Construction of Bovine Sex Related Genes FOXL2 Standard Plasmid and Standard Curve by Real-time RT-PCR[J]. , 2012, 39(1): 1-5.

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Construction of Bovine Sex Related Genes FOXL2 Standard Plasmid and Standard Curve by Real-time RT-PCR

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