Abstract: To search for differentially expressed genes and analysis its genetic effections,in this study,actin alpha 1 (ACTA1) gene was found differentially expressed in longissimus dorsi of Yanbian Yellow cattle,which had significant difference on intramuscular fat (IMF) content, and its full-length cDNA sequence was obtained from Yanbian Yellow cattle. The tissue expression analysis indicated that the ACTA1 gene was highly expressed in muscle and longissimus dorsi. One SNP was detected by sequence comparison in 5' non-coding region.The polymorphisms of ACTA1 were detected in a 100 Yanbian Yellow cattles population by PCR-RFLP and the association of genotype with growth traits,which result in 3 genotypes named AA,AG and GG respectively,the genotype frequency of AA,AG and GG were 0.24,0.50 and 0.26;Allele frequencies of A and G were 0.37 and 0.63. Variance analysis showed: some indexes of the individuals with GG genotype were higher than that with genotype AA and AG, such as the indexes of body length, body weight and average day gain(P＜0.05); the heart girth of individuals with GG genotype was extremely higher than AG genotype (P<0.05); there were not significantly differences in the wither height individuals with different genotype.The GG genotype may have positive effect on growth traits. This indicated that ACTA1 gene as marker can be used to selection of growth trait in Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; ACTA1 gene; growth trait