猪繁殖与呼吸综合征RT-LAMP检测方法研究

›› 2011, Vol. 38 ›› Issue (6): 136-141.

• 疾病防治 • Previous Articles Next Articles

Development of RT-LAMP for Detecting Porcine Reproductive and Respiratory Syndrome Virus

YANG Su¹, SHA Cai-hua¹, GU Hai-yang², DONG Shang-zhi³, LIAO Xiu-yun¹, XU Hai-nie¹, BO Qing-ru¹, LUO Bao-zheng¹, CHEN Wei-sheng¹

1. Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519015, China;2. Tarim University, Alaer 843300, China;3. Zhuhai Sea Agriculture Fishing Water Utilities Bureau, Zhuhai 519000, China

Received:2010-11-03 Revised:1900-01-01 Online:2011-06-20 Published:2011-06-20

Abstract

Abstract: Applying reverse transcription LAMP (RT-LAMP), according to the N protein conserved sequence of PRRSV ORF5 genes from six regions, two sets of primers were designed. By optimizing reaction system and conditions, the RT-LAMP method for detecting PRRSV was developed after sensitivity and specificity tests. The research results indicate that PRRSV nucleic acids amplification can be completed within 1 h in the isothermy condition of 63 ℃ only using a common constant temperature water-bath, test results can be judged with naked eyes after adding nucleic acids dye SYBR GreenⅠ. It provided a kind of cheap, simple, speedy, high sensitivity and specificity PRRSV detecting method for work field and grass-roots units.

Key words: porcine reproductive and respiratory syndrome virus; RT-LAMP; detection method; research

CLC Number:

S852.65
Q78

YANG Su;SHA Cai-hua;GU Hai-yang;DONG Shang-zhi;LIAO Xiu-yun;XU Hai-nie;BO Qing-ru;LUO Bao-zheng;CHEN Wei-sheng. Development of RT-LAMP for Detecting Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2011, 38(6): 136-141.

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Development of RT-LAMP for Detecting Porcine Reproductive and Respiratory Syndrome Virus

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