GnRH脉冲对体外原代培养大鼠GTH细胞中 LHβ mRNA表达水平的影响

China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (4): 55-58.

• 生理生化 • Previous Articles Next Articles

The Influence of GnRH Impulse on the Expression of LHβ mRNA in Rats’ GTH Cells Primarily Cultured in vitro

WANG Xin^1,2, TAN Jian-hua³, LAI Xiao-ping², WAN Zhong-hai³, WEI Xu-bin⁴

1. College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;2. Traditional Chinese Medical Academy,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;3. Institute of Military Veterinary Academy of Military Medical Science,PLA,Changchun 130062,China;4. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China

Received:2010-10-11 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract

Abstract: To explain at molecular level the influence of GnRH at different impulse frequencies on the secretion of LH by GTH.Research the change of LHβ mRNA in cells when the pituicytes of original generation of rats cultured in vitro were stimulated by GnRH at different impulse frequencies, by applying the technique of culture in vitro of cells in combination with the fluorescent quantitation of PCR.The result showed that in the condition of the same vibration amplitude, when the cells were stimulated by impulse at high frequency (at the interval of 30 min), the level of LHβ mRNA reached the top.This supports the hypothesis that GnRH impulse frequency itself is a regulation signal,and GnRH impulse at high frequency brings about mainly the response of LHβ.

Key words: GnRH; GTH; LHβ mRNA

CLC Number:

S852.2

WANG Xin;TAN Jian-hua;LAI Xiao-ping;WAN Zhong-hai;WEI Xu-bin. The Influence of GnRH Impulse on the Expression of LHβ mRNA in Rats’ GTH Cells Primarily Cultured in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(4): 55-58.

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The Influence of GnRH Impulse on the Expression of LHβ mRNA in Rats’ GTH Cells Primarily Cultured in vitro

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