不同培养基对小鼠黄体细胞体外培养效果的影响

China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (4): 59-61.

• 生理生化 • Previous Articles Next Articles

The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro

CHEN Juan¹, YANG Yong-mei¹, CHEN Yang¹, JIANG Huai-zhi¹, YANG Yu-jiang¹, GONG Jun-ming²

1. College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2. The Animal Husbandry of Shuangliao,Shuangliao 136400,China

Received:2010-09-21 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract

Abstract: Female mice were injected with PMSG and HCG before sexual maturity, removed the ovaries to culture ovarian corpus luteum cells in vitro at the appropriate time. The two kinds of culture medium were DMEM with 10% serum and RPMI 1640 with 10% serum. The results showed that the former medium was better than the latter in shape, volume and quantity of corpus luteum cells. This provided a reference for selecting culture solution to culture ovarian corpus luteum cells in vitro and the further research.

Key words: medium; corpus luteum; culture in vitro

CLC Number:

Q813.1⁺1

CHEN Juan;YANG Yong-mei;CHEN Yang;JIANG Huai-zhi;YANG Yu-jiang;GONG Jun-ming. The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(4): 59-61.

[1]	JIA Yuchen, JIANG Hui, LAN Xiner, LI Jing, ZHU Yiping. Advances in Mesenchymal Stromal Cell-Derived Cell-Free Therapies for Treating Equine Osteoarthritis [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1924-1931.
[2]	MA Linli, XU Guoqiang, ZHANG Qiuping, CHE Qiaolin, SHI Jinsong, XU Zhenghong. Study on the Optimization of Medium Composition to Increase the Viable Counts of Streptococcus suis Type 2 NT15B Strain [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2776-2786.
[3]	LIU Fang, QUAN Tingting, ZHANG Fei, SHI Liqiao, LIU Manli, WU Zhaoyuan. Optimization of Streptomyces sp. Fermentation Medium for Production of Alkylated Anthraquinone by Response Surface Methodology [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 3142-3154.
[4]	YE Jinling, FAN Qiuli, WANG Yibing, LIN Xiajing, RUAN Dong, LUO Qili, GOU Zhongyong, JIANG Shouqun. Effects of Phospholipids and Soybean Isoflavones on Growth Performance, Meat Quality,and Antioxidant Function of Yellow-feathered Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 1857-1867.
[5]	LIU Bo, LI Meilin, LIU Xu, LIANG Shuangying, MU Baolong, ZHAO Wenting, GE Yansong. Effects of Adipose-derived Stem Cell Conditioned Medium Alleviates Oxidative Stress Injury Induced by Sodium Taurocholate and Trypsin [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(2): 798-806.
[6]	PENG Zhiping, ZHANG Jingyan, GUO Zhiting, WANG Lei, ZHANG Kai, ZHANG Kang, WANG Guibo, QIU Zhengying, WANG Xuezhi, LI Jianxi. Extraction and Content Detection of Cordycepin from Cordyceps militaris Residual Medium by Hydrothermal Reflux Method [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(9): 3333-3342.
[7]	ZHANG Yan, XUE Zhanmei, LIU Xuesong, SU Jing, HAO Jingyou, TANG Wei, LIU Yankai, SUN Hongjie, SHI Tongrui. Study on Components of Solid-state Fermentation Medium of Qingfei Granules [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(3): 1162-1171.
[8]	FENG Xin, ZHANG Luomeng, LUAN Jiaming, ZHOU Jinying, ZHANG Min, GENG Chunyin. Advance on Application and Influence Factors of Medium Chain Fatty Acids in Animal Production [J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(6): 1739-1749.
[9]	WANG Xiaodong, YANG Chan, LIU Qinghua, LI Zhenyu, WU Hao, LIU Guoshi, LI Xiang, HE Changjiu. The Construction of the Goat Ovary Melatonin Signal Specific Vectors pcDNA3.1-Pro/GDF9-ASMT and pcDNA3.1-Pro/Cyp17-MTNR1A and MTNR1B [J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(12): 3833-3843.
[10]	ZHANG Zhiyan, ZHAO Qian, YU Jiamin, LIU Changqing, XU Haiyan, GU Wei. Selection of Culture Medium and Optimization of Fermention Conditions for Bacillus subtilis Producing Antimicrobial Peptides [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(4): 1217-1226.
[11]	SONG Zi-yu, FAN Chao-yue, ZHONG You-gang. Research Progress on Regulatory Mechanisms of Canine Corpus Luteum Function [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(9): 2598-2602.
[12]	HUANG Deng-gui, LIAO Wen-de, WEN Yuan-kun, ZHUANG Yue-feng, LIU Guo-qiang, HUANG Xiao-liang, HUANG Guan-qing. Effects of Medium-chain Triglyceride on Growth Performance and Serum Biochemical Indices in Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(7): 1761-1765.
[13]	LIU Xian-xia, WANG Han-kui, WANG Shu-jie, WANG Hai-bin, GU Xin-li. Analysis of B-ultrasound Luteal Variation Related Indicators of Delayed Ovulation Dairy Cows after Estrus at Different Stages [J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(4): 991-998.
[14]	LUO Hai-yan, DOU Bing-ran, JIANG Kai-wei, HONG Hou-sheng. Research Progress on Technology of Fed-batch in Animal Cell Culture [J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(1): 176-181.
[15]	GU Yu-feng, HUANG Ya-jun, WANG Qin, WU Yong, LIU Zhao-ying, ZHANG Ming-jun, SUN Zhi-liang, JIANG Zheng-yun. Study on the Extraction Process of Compound Epimedium by Orthogonal Experimental Design [J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(3): 255-258.

The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments