不同培养基对小鼠黄体细胞体外培养效果的影响

›› 2011, Vol. 38 ›› Issue (4): 59-61.

• 生理生化 • Previous Articles Next Articles

The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro

CHEN Juan¹, YANG Yong-mei¹, CHEN Yang¹, JIANG Huai-zhi¹, YANG Yu-jiang¹, GONG Jun-ming²

1. College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2. The Animal Husbandry of Shuangliao,Shuangliao 136400,China

Received:2010-09-21 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract

Abstract: Female mice were injected with PMSG and HCG before sexual maturity, removed the ovaries to culture ovarian corpus luteum cells in vitro at the appropriate time. The two kinds of culture medium were DMEM with 10% serum and RPMI 1640 with 10% serum. The results showed that the former medium was better than the latter in shape, volume and quantity of corpus luteum cells. This provided a reference for selecting culture solution to culture ovarian corpus luteum cells in vitro and the further research.

Key words: medium; corpus luteum; culture in vitro

CLC Number:

Q813.1⁺1

CHEN Juan;YANG Yong-mei;CHEN Yang;JIANG Huai-zhi;YANG Yu-jiang;GONG Jun-ming. The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro[J]. , 2011, 38(4): 59-61.

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The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro

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