牙釉质基因鉴定山羊性别的研究

›› 2011, Vol. 38 ›› Issue (4): 162-165.

• 遗传繁育 • Previous Articles Next Articles

Sex Identification in Goat by Amplification of Amelogenin (AMEL) Gene

TANG Ji-wei^1,2, ZHANG Zhi-guo³, GAO Qing-hua², SHEN Bo^1,2, WU Yan-feng²

1. College of Life Science, Tarim University, Alar 843300,China;2. Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production and Construction Groups, Alar 843300,China;3. Dalian Grgnd Foundation Breeding Livestock Co.Ltd., Dalian 116600,China

Received:2010-09-13 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract

Abstract: Based on the characteristic of the hircine AMEL-X and AMEL-Y gene with various degree deletions,a pair of primers were utilized to establish the process of sex identification by PCR and agarose gel electrophoresis with mixed samples of the hircine blood genomic DNA, and the PCR produced was purified, cloned and sequenced, then sex identification to the 46 hircine blood samples(♀:24,♂:22).The results showed that the male hircine sample produces a non-specificity band and a specificity band, while the female hircine sample produces one band. The non-specificity fragment 349 bp, the specificity fragment 289 bp. By comparison, the results of the 46 hircine blood DNA samples sex identification with the hircine actual sex, the female accuracy 100%(22/22), the male accuracy 100%(24/24).

Key words: amelogenin(AMEL) gene; goat; PCR; sex identification

CLC Number:

Q344⁺.2

TANG Ji-wei;ZHANG Zhi-guo;GAO Qing-hua;SHEN Bo;WU Yan-feng. Sex Identification in Goat by Amplification of Amelogenin (AMEL) Gene[J]. , 2011, 38(4): 162-165.

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Sex Identification in Goat by Amplification of Amelogenin (AMEL) Gene

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