蛇四棘食道口线虫线粒体cox1 基因的克隆及序列分析

Abstract

Abstract: To analyze the differences in cytochrome c oxidase subunit 1 (cox1) gene of Oesophagostomum quadrispinulatum isolated from Changsha city. The partial cox1 (pcox1) was amplified from each O.quadrispinulatum sample,and pcox1 sequences were aligned using the Clustal X 1.83. Sequence homology analysis was performed using the MegAlign program of the software DNAStar version 5.0 by comparing with the corresponding sequence of O.quadrispinulatum available in GenBank^TM. The results showed that the lengths of pcox1 were 393 bp. Sequence comparison revealed that the similarity in pcox1 sequences among Changsha isolates and that of the O.quadrispinulatum available in GenBank^TMwere over 98%. There was no significant variation in pcox1 sequences within O.quadrispinulatum,while inter-species difference was obviously.The results provided a foundation for further studies of molecular identification and molecular genetics of O.quadrispinulatum.

Key words: Oesophagostomum quadrispinulatum; cox1 gene; sequence analysis

CLC Number:

CHEN Wen-cheng;XU Ping-yuan;LI Fen;LIU Wei;LIU Yi . Cloning and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 1 of Oesophagostomum quadrispinulatum Isolates from Changsha City[J]. , 2011, 38(4): 113-116.

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Cloning and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 1 of Oesophagostomum quadrispinulatum Isolates from Changsha City

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