Abstract: In the mid-1990s, the Luminex Corporation(USA) exploited the first suspension array system, which used fluorescent-coded carboxylic beads as solid carrier. Proteins, oligonucleotides, small peptides and lipids have been adsorbed or chemically coupled to the surface of microspheres to format detected probe. According to the different proportion of red and orange fluorescent dyes inside beads, the beads are divided into 100 different types. The intensity of fluorescent associated with beads and fluorescent probe are tested simultaneously by special detector on suspension array system. The detector can distinguish beads of different types basing on different proportion of two fluorescent dyes while recording the intensity of fluorescent from probe on every bead to achieve high throughput detection. This article reviews the research regarding suspension assay system and its application in detection.

Key words: suspension array system; protein; nucleotide; detection