›› 2011, Vol. 38 ›› Issue (2): 239-242.

• 经验交流 • Previous Articles     Next Articles

Determination of p-coumaric Acid(PCA) in PCA Solution by RP-HPLC〖

ZHOU De-gang1,HE Cheng2,SUN Zhi-wen1,LI Ying-chao1   

  1. (1.Beijing Institute of Veterinary Drug Control，Beijing 100107,China;2.Key Laboratory of Evaluation and Innovation of Veterinary Medicine and Chinese Herbal Medicine of Ministry of Agriculture，China Agricultural University，Beijing 100193, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20

Abstract: It was aimed to establish high performance liquid chromatography（HPLC）method for determination of p-coumaric acid (PCA) in PCA solution. The p-coumaric acid was successfully separation performed on XTerra〖KG-*4〗〖XC1.TIF〗RP18 column at ambient temperature，MeOH-1% acetic acid (40∶60) was the mobile phase，the detection wavelength was 310 nm and flow-rate 1 mL/min. The results showed that there was good liner relationship(r＝0.999904) in the range of 2 to 500 μg/mL，RSD＝0.5%，the average recovery rate was 98.22% to 101.07%. The results showed that the simple，rapid and accurate method could be used for the quality control of PCA solution.

Key words: p-coumaric acid(PCA); reversed-phase high performance liquid chromatography（RP-HPLC）; determination

CLC Number: