›› 2011, Vol. 38 ›› Issue (2): 132-135.

• 遗传繁育 • Previous Articles     Next Articles

Study on IGFⅡ Gene SNP and its Effect on Meat Traits in Jilin White Goose

XU Jing1,2,SHAN Xue-song2   

  1. (1.Development Institute of Jilin Agricultural University, Changchun 130600, China;2.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
  • Contact: SHAN Xue-song

Abstract: Five primers were designed to amplify IGFⅡ gene and SNPs were detected by PCR-SSCP in 85 Jilin white goose meat stock population. The population genetic construction and the correlation between SNPs and carcass traits were analyzed. The results of PCR-SSCP showed that there were 3 genotypes of AA, AB, BB in exon 3 region of IGFⅡ, the genotypic frequency were 0.7647, 0.1412 and 0.0941, respectively,AA was dominance genotypes. There were 2 allele genes of A,B, gene frequency were 0.8353 and 0.1647, respectively, A was dominance allele gene. One SNP was found in
exon 3 region of IGFⅡ (A→G), sequence analogy indicated that it was a silent mutation, and the amino sequence was not change. By variance analysis for three genotypes, AA, AB genotypes were significant lower than BB genotype for half wipe chest weight and liver weight (P<0.05), AA genotype were significant lower than BB genotype for leg weight (P<0.01). There were no significant level for whole wipe chest, chest weight and wing weight(P>0.05).

Key words: goose; IGFⅡ; SNP; meat trait

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