运用SYBR Green Ⅰ熔解曲线法检测饲料中牛、羊源性成分的研究

Abstract

Abstract: DNA melting curve analysis of SYBR Green Ⅰ was to be established as a method for the detection of dovine and sheep derived materials in feed. SYBR Green Ⅰ was used as fluorescent. The sensitivity was improved by optimizing the reaction system. Comparing with the method of GB/T20190-2006, the accuracy of the method of DNA melting curve analysis was 100%. The method of DNA melting curve analysis could be used for the dectetion of bovine and sheep derived materials in feed.

Key words: bovine and sheep derived materials; SYBR Green Ⅰ; fluorescent; melting curve

CLC Number:

S786

BAN Fu-guo;LIU Hong-wei;GAO Yan-ling;MA Jun. Research of SYBR Green Ⅰ Melting Curve Detection for Derived Materials in Feed in Bovine and Sheep[J]. , 2011, 38(2): 128-131.

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Research of SYBR Green Ⅰ Melting Curve Detection for Derived Materials in Feed in Bovine and Sheep

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