›› 2011, Vol. 38 ›› Issue (2): 103-106.

• 生物技术 • Previous Articles     Next Articles

Sequence and Analysis of Partial Mitochondrial nad1Gene for Ascaridia galli Isolates from Hunan Province

ZHANG Jin1,2,LIU Yi1,ZOU Jian-zeng2   

  1. (1.College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128,China;2.Supervision of Animal Epidemic Prevention Station of Hunan, Changsha 410013, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20

Abstract: To analyze the differences of the NADH dehydrogenase subunit 1(nad1) gene of Ascaridia galli strains from Hunan province, the partial nad1(pnad1) was amplified from each Ascaridia galli sample, and pnad1 sequences were aligned using the Clustal X 1.81. The lengths of all the pnad1 sequences was 408 bp. There was no significant variation in pnad1 sequences within Ascaridia gall, while inter-species difference was obvious, so pnad1 sequences could be used as genetic marker for population genetic studies of roundworms. The results of this study lay down the foundation for further study on molecular epidemiology and diagnostics of Ascaridia galli.

Key words: Ascaridia galli; mitochondrial DNA; nad11 gene

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