›› 2011, Vol. 38 ›› Issue (12): 226-231.

• 经验交流 • Previous Articles     Next Articles

Study on the Quality Standard of Huangbai Oral Liquid

WANG Sheng-yi1, QI Zhi-ming1, LIU Zhi-qi2, LI Xia1, DONG Shu-wei1, LIU Shi-xiang1, LIU Yong-ming1   

  1. 1. Engineering &Technology Research Center of TCVM of Gansu Province,Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture,Lanzhou Institute of Animal Science and Veterinary Pharmaceutics Chinese Acadmey of Agricutural Sciences,Lanzhou 730050,China;2. College of Life and Engineering,Lanzhou University of Technology, Lanzhou 730050,China
  • Received:2011-04-14 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract: To establish a quality control standard for Huangbai oral liquid,Rhizoma coptidis, Flos lonicerae ,Radix scutellariae and charred radix sanguisorbae were identified by TLC. Content of Berberine hydrochloride and Baicalin were determinated by HPLC. The results showed that the TLC identification was highly specific and the spots were clear and focused with good reproducibility . The calibration curve of Berberine hydrochloride was linear in the range of 0.139 to 0.834 μg(R2＝0.9999). The average recovery rate was 97.04%, RSD＝0.97%(n＝6). The calibration curve of Baicalin was linear in the range of 0.235 to 1.41 μg(R2＝0.9999). The average recovery rate was 97.64% , RSD＝0.71%(n＝6). The quality of the preparation could be controlled by qualitative identification of Coptis, honeysuckle, Scutellaria , Sanguisorba carbon and content determination of Berberine hydrochloride and Berberine.

Key words: huangbai oral liquid; TLC; HPLC; quality criteria

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