五华鸡J亚群白血病病毒env部分基因的克隆和序列分析

Abstract

Abstract: The partial sequence of avian leukosis virus subgroup J (ALV-J) env gene was cloned by PCR in Anhui province, which was named as ALV-J-env1. And we compared ALV-J-env1 with the partial sequence of eight ALV-J env genes in the GenBank database. The results showed that ALV-J infection in Wuhua chicken was found in Anhui province. Alignment of nine gene sequences of ALV-J env, the homology between ALV-J-env1 and the other sequences ranged from 94.2% to 96.6%. The result revealed that the env gene from different strains had the mutation at nucleotide sequence. However, the highest homology with ALV-J-env1 were HQ425636 and HM235665 gene sequences isolated from strains in China, and they gathered together in the evolutionary tree, indicating a close genetic relationship, and they evolved from common strains. The lowest homology with ALV-J-env1 was the gene sequence of AY312965 isolated from Malaysia, it could be further confirmed by phylogenetic analysis.

Key words: avian leukosis virus subgroup J; partial env gene; sequence analysis

CLC Number:

DAI Yin;ZHANG Dan-jun;HU Xiao-miao;ZHAO Rui-hong. DNA Cloning and Sequence Analysis of Partial env Gene of Avian Leukosis Virus Subgroup J in Wuhua Chicken Flocks[J]. , 2011, 38(12): 122-124.

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DNA Cloning and Sequence Analysis of Partial env Gene of Avian Leukosis Virus Subgroup J in Wuhua Chicken Flocks

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