›› 2011, Vol. 38 ›› Issue (10): 106-112.

• 遗传繁育 • Previous Articles     Next Articles

Genetic Diversity of Microsatellite DNA in Nine Sheep Breeds

LOU Yuan-gen1,2, LIU Chou-sheng2, LIU Jian-xue1, LIU Gang2, HAN Xu2   

  1. 1. College of Food & Bioengineering,Henan University of Science and Technology,Luoyang 471003,China;2. National Center for Preservation and Utilization of Genetic Resources of Domestic Animals and Forages, National Animal Husbandry and Veterinary Service, Beijing 100193,China
  • Received:2011-02-16 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

Abstract: The genetic diversity of nine Chinese indigenous sheep breeds were analyzed with fluorescence PCR using 10 microsatellite markers recommended by the FAO and ISGA. The results showed that 153 alleles were detected on 10 microsatellite loci, the effective number of which ranged from 2.3064 to 13.7632,and the loci polymorphism information content and heterozygosity varied from 0.5395 to 0.8559 and from 0.3972 to 0.8475, with locus genetic differentiation in the range from 0.042 to 0.149. The conclusion of the genetic structure relationship and DA / UPGMA dendrogram and MVSP cluster analysis diagram was basically consistent with sheep origin and evolution and genetic relationship and geographic distribution.

Key words: sheep; microsatellite; genetic diversity

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