Abstract: In this study, 4 mL canine distemper virus being 104.5 TCID50/0.1 mL, was used to immunize wusuli-raccoon dog which was strain economic animal. After 10 days, animal blood was collected, while blood serum was disassociated. Neutralization test was made to evaluate the potency of antibody, during this test, virus was 200TCID50 , blood serum whose volume was equal with virus was diluted by times after times, while immunoglobulin was extracted crudely by saturated ammonium sulfate. The results clearly suggested that under the project that the blood serum was diluted for 2917 times, the immunoglobulin which was extracted crudely by saturated (NH3)2SO4 was diluted for 2344 times, there was 50% Vero cytoprotection without CPE. 24 white raccoon dogs with this disease were therapied by the whole serum above, and 22 ones was survival, therefore,we demonstrated that antiserum, made from strain animal such as fox, could be used tocure canine distemper virus.

Key words: canine distemper virus; antiserum; therapy