穴位埋线对运动性疲劳大鼠能量代谢水平的影响

doi:10.16431/j.cnki.1671-7236.2021.02.037

Abstract

Abstract: The purpose of this trial was to explore the effect of thread-embedding therapy on exercise-induced fatigue.The exercise-induced fatigue rats were taken as the research object.36 SD rats were randomly divided into blank group,model group and treatment group,with 12 rats in each group.Thread-embedding therapy was performed on the treatment group before training,to study thread-embedding therapy for regulating energy metabolism in exercise-induced fatigue.After a 7-week training,except for the blank group,the exercise groups performed high-speed exhaustion exercises.The exercise time,energy metabolism-related indicators and fatigue-related biomarkers were measured and skeletal muscle sections were made.The results showed that compared with control group,the blood glucose and glycogen contents of the exercise groups were significantly reduced (P<0.05),and the contents of lactic acid,urea nitrogen and malondialdehyde were significantly increased (P<0.05).Pathological damage was observed under light microscopy of skeletal muscle sections.The ATP content and ATPase activity of the treatment group were significantly higher than those of the model group (P<0.05),and the increase in creatine kinase content was lower than that of the model group.In summary,these results indicated that thread-embedding therapy made the body have more energy substances and fewer metabolites,and might regulate the body's energy metabolism level by improving oxidative stress and increasing energy supply,and delay the generation of exercise-induced fatigue.

Key words: thread-embedding therapy; exercise-induced fatigue; energy metabolism; skeletal muscle; rat

CLC Number:

S853.61

SHI Xiaoyu, SONG Yue, FENG Min, GAO Zhenzhen, YANG Ying. Thread-embedding Therapy for Regulating Energy Metabolism in Exercise-induced Fatigue[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 747-754.

References

[1] 王亚雯,辛中豪,高蔚娜,等.植物来源化合物抗疲劳作用研究进展[J].军事医学,2016,40(12):1009-1012. WANG Y W,XIN Z H,GAO W N,et al.Advances in research on anti-fatigue effect of phytochemicals[J].Military Medical Sciences,2016,40(12):1009-1012.(in Chinese)
[2] LAMBERT E V,NOAKES T D,HAMPSON D B,et al.Exercise and fatigue-control mechanisms[J].International Sportmed Journal,2001,2(3):1-14.
[3] FINAUD J,LAC G,FILAIRE E.Oxidative stress relationship with exercise and training review[J].Sports Medicine,2006,36(4):327-358.
[4] 佟强,曹建民,周海涛.雨生红球藻对过度训练大鼠骨骼肌MAPK信号通道蛋白表达及抗氧化能力的影响[J].中国体育科技,2016,52(4):133-138. TONG Q,CAO J M,ZHOU H T.Effects of Haematococcus pluvialis on mitogen-activated protein kinase signaling pathways protein expression and antioxidant capacity in overtraining rat skeletal muscle[J].China Sport Science and Technology,2016,52(4):133-138.(in Chinese)
[5] 郑秀琴,刘文珂,阮明玉,等.针灸对运动性疲劳的能量代谢调节作用述评[J].广州中医药大学学报,2019,36(4):532-536. ZHENG X Q,LIU W K,RUAN M Y,et al.Review of acupuncture and moxibustion therapy for regulating energy metabolism in exercise-induced fatigue[J].Journal of Guangzhou University of Traditional Chinese Medicine,2019,36(4):532-536.(in Chinese)
[6] 魏玉婷,曹朝霞,李小娟,等.穴位埋线疗法的分子生物学机制研究进展[J].中华中医药杂志,2019,34(8):3633-3636. WEI Y T,CAO Z X,LI X J,et al.Research progress in the molecular biological mechanisms for implanting catgut in acupoint therapy[J].China Journal of Traditional Chinese Medicine and Pharmacy,2019,34(8):3633-3636.(in Chinese)
[7] 罗永江,郑继方,辛蕊华.比较针灸学[M].第2版.北京:中国农业出版社,2017. LUO Y J,ZHENG J F,XIN R H.Comparative Acupuncture and Moxibustion[M].2nd Edition.Beijing:China Agriculture Press,2017.(in Chinese)
[8] 中国国家标准化管理委员会.中华人民共和国国家标准:针灸技术操作规范:北京:第10部分穴位埋线:GB/T21709.10-2008[S].北京:中国标准出版社,2008. STANDARDIZATION ADMINISTRATION.National standards of People's Republic of China:Standardized manipulations of acupuncture and moxibustion-Part 10:Thread-embedding therapy:GB/T21709.10-2008[S].Beijing:Standards Press of China,2008. (in Chinese)
[9] 纪树荣.康复医学[M].北京:高等教育出版社,2004. JI S R.Rehabilitation Medicine[M].Beijing:Higher Education Press,2004.(in Chinese)
[10] 杨道宁,李丽.运动性疲劳动物模型制备的研究进展[J].沈阳体育学院学报,2011,30(3):80-85. YANG D N,LI L.A summarizing study on preparation of animal model of exercise-induced fatigue[J].Journal of Shenyang Sport University,2011,30(3):80-85.(in Chinese)
[11] 朱浩,姜梦如,马晓宇,等.穴位埋线对运动性疲劳模型大鼠肝肾功能的影响[J].中国兽医学报,2017,37(3):501-504. ZHU H,JIANG M R,MA X Y,et al.Influence of point embedding therapy on the liver and kidney functions of sports fatigue rats[J].Chinese Journal of Veterinary Science,2017,37(3):501-504.(in Chinese)
[12] 杜宁宇.针刀干预对触发点模型大鼠肌肉ATP、肌糖元和乳酸含量的影响[D].北京:北京中医药大学,2017. DU N Y.Effect of needle-knife intervention on muscle ATP,muscle glycogen and lactic acid content of trigger point model rats[D].Beijing:Beijing University of Chinese Medicine,2017.(in Chinese)
[13] 刘瑄,窦思东,易红梅,等.经皮穴位电刺激防治运动性疲劳的研究进展[J].广州中医药大学学报,2015,32(3):566-568. LIU X,DOU S D,YI H M,et al.Advances in the research on prevention and treatment of sports fatigue by percutaneous electrical stimulation[J].Journal of Guangzhou University of Traditional Chinese Medicine,2015,32(3):566-568.(in Chinese)
[14] 游世晶,夏勇,李艺,等.经皮穴位电刺激对大运动量耐力训练大鼠骨骼肌能量代谢的影响[J].上海针灸杂志,2011,30(3):192-194. YOU S J,XIA Y,LI Y,et al.Effect of transcutaneous electrical acupoint stimulation on skeletal muscle energy metabolism in strenuous endurance training rats[J].Shanghai Journal of Acupuncture and Moxibustion,2011,30(3):192-194.(in Chinese)
[15] JACKMAN S R,WITARD O C,JEUKENDRUP A E,et al.Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise[J].Medicine & Science in Sports & Exercise,2010,42(5):962.
[16] RODRIGUES B M,DANTAS E,DE SALLES B F,et al.Creatine kinase and lactate dehydrogenase responses after upper-body resistance exercise with different rest intervals[J].Journal of Strength & Conditioning Research,2010,24(6):1657.
[17] 朱洪竹,朱梅菊,蔡荣兴,等.毛蕊花苷对大鼠递增大强度运动下骨骼肌损伤的保护作用研究[J].山东体育学院学报,2019,35(1):53-57. ZHU H Z,ZHU M J,CAI R X,et al.Protective effect of verbascoside on skeletal muscle injury under high-intensity in increasing trained rats[J].Journal of Shandong Sport University,2019,35(1):53-57.(in Chinese)
[18] 曹庆雷,罗明刚.运动性疲劳生物标志物的研究进展[J].四川体育科学,2017,36(5):47-52. CAO Q L,LUO M G.Advances in research on biomarkers of exercise induced fatigue[J].Sichuan Sports Science,2017,36(5):47-52.(in Chinese)
[19] 关宇,巫苗苗,赵宁宁,等.重复性大强度运动大鼠在不同时期肌糖原的变化规律[J].北京体育大学学报,2005(6):769-770. GUAN Y,WU M M,ZHAO N N,et al.Research on the variation principles of the muscle glycogen in rats during repeated bouts of high intensity exercise[J].Journal of Beijing Sport University,2005(6):769-770.(in Chinese)
[20] 龚云.运动疲劳对大鼠纹状体神经元GLUT_3 mRNA和蛋白表达的影响[J].中国运动医学杂志,2016,35(11):1025-1030. GONG Y.Effect of sports fatigue on the expression of GLUT_3 mRNA and protein in striatal neurons of rats[J].Chinese Journal of Sports Medicine,2016,35(11):1025-1030.(in Chinese)
[21] 唐汉庆,陈桦,韦祎,等.铁皮石斛对运动疲劳小鼠能量代谢和CNTF mRNA表达的影响[J].中国实验方剂学杂志,2014,20(15):164-167. TANG H Q,CHEN H,WEI Y,et al.Effects of dendrobium officinale on energy metabolism and expression of CNTF mRNA in athletic fatigue mice[J].Chinese Journal of Experimental Tradi-tional Medical Formulae,2014,20(15):164-167.(in Chinese)
[22] BÖNING D,MAASSEN N.Relation between lactic acid and base excess during muscular exercise[J].European Journal of Applied Physiology,2018,118(4):863-864.
[23] 张沙骆,王丹,王芳.茶多酚对力竭运动大鼠骨骼肌组织中细胞凋亡的影响[J].中国老年学杂志,2019,39(14):3532-3535. ZHANG S L,WANG D,WANG F.Effect of tea polyphenols on apoptosis in skeletal muscle tissue of exhausted exercise rats[J].Chinese Journal of Gerontology,2019,39(14):3532-3535.(in Chinese)
[24] 陈万,田诗彬,吴春燕,等.运动疲劳恢复期不同时相大鼠丙二醛含量、超氧化物歧化酶活性及相关指标动态变化特征[J].中国康复医学杂志,2013,28(11):1001-1005. CHEN W,TIAN S B,WU C Y,et al.Dynamic changing features of MDA,SOD and related parameters in rats during different phases of recovery period after exhaustive running[J].Chinese Journal of Rehabilitation Medicine,2013,28(11):1001-1005.(in Chinese)
[25] LIANG Y,FANG J Q,WANG C X,et al.Effects of transcutaneous electric acupoint stimulation on plasma SOD and MDA in rats with sports fatigue[J].Acupuncture Research,2008,33(2):120-123.
[26] 郭文,罗学港,王慧,等.降钙素基因相关肽对大鼠运动性疲劳的影响[J].体育科学,2016,36(7):63-70. GUO W,LUO X G,WANG H,et al.Effects of calcitonin gene-related peptide on the exercise-induced fatigue of rats[J].China Sport Science,2016,36(7):63-70.(in Chinese)
[27] 黄诚胤,潘建华,李慧.表没食子儿茶素没食子酸酯对小鼠运动疲劳的拮抗作用[J].中国应用生理学杂志,2015,31(1):85-88. HUANG C Y,PAN J H,LI H.Effect of epigallocatechin gallate against exercise-induced fatigue in mice[J].Chinese Journal of Applied Physiology,2015,31(1):85-88.(in Chinese)
[28] 赵甜甜.紫甘薯花青素对过度训练大鼠骨骼肌氧化损伤保护作用的研究[D].徐州:江苏师范大学,2013. ZHAO T T.Study on the protective effect of anthocyanin from purple sweet potato on the oxidative injury in overtrained rats' skeletal muscle[D].Xuzhou:Jiangsu Normal University,2013.(in Chinese)
[29] 魏源,方春露,李良鸣,等.延迟性肌肉酸痛动物模型的建立及组织形态学观察(英文)[J].中国组织工程研究,2015,19(29):4645-4651. WEI Y,FANG C L,LI L M,et al.Establishing an animal model of delayed onset muscle soreness and its histomorphologic observation[J].Chinese Journal of Tissue Engineering Research,2015,19(29):4645-4651.

Thread-embedding Therapy for Regulating Energy Metabolism in Exercise-induced Fatigue

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Guitao, MA Jideng, LI Xuewei, GE Liangpeng, ZHANG Jinwei. Research Advance on Regulation of Skeletal Muscle Physiology Function by Short Chain Fatty Acids [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2286-2295.
[2]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[3]	LI Can, LI Chuchu, ZENG Wei, ZHANG Ning, JI Chunxiao, CHEN Tao. Recombinant Expression and Function Study of RegⅢγ Protein in Porcine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2414-2426.
[4]	CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.
[5]	LIU Peng, LI Jingjing, QIAO Yanliang, ZHU Lianqin, ZHU Fenghua. Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2518-2530.
[6]	NIU Yeyu, SHEN Qi, BAI Yong, SUN Panpan, SUN Na, YIN Wei, FAN Kuohai, LI Hongquan, SUN Yaogui. Anti-inflammatory and Antioxidative Effects of Extracts of Physalis Calyx seu Fructus Based on Ulcerative Colitis Model [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2531-2539.
[7]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, LIU Fei, YU Debing. Sequencing Analysis of the Full-length Transcriptome of Granulosa Cells of Small Yellow Follicle in Hy-Line Variety Brown [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1754-1763.
[8]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[9]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[10]	SONG Chenglei, ZHANG Zhe, WEI Yilin, YANG Songbai, DOU Yaqing, LI Chenlei, LIU Yingke, LI Xinjian, YANG Feng, LI Xiuling, WANG Kejun, QIAO Ruimin, HAN Xuelei. Effects of Different Breeds,Seasons and Month Ages on Semen Quality of Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1938-1946.
[11]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[12]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[13]	ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.
[14]	TANG Yu, YANG Yifeng, ZHANG Ying, XUE Hailong, FENG Huailiang, XU Baozeng. Cytological Study on Oocyte Maturation of Raccoon Dog [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1434-1443.
[15]	WANG Leping, PAN Yan, TAN Yizhou, LI Lei, HAN Kaiou, CAO Yajie, LI Jun, ZHOU Yuqing, LIAO Yuying, MA Dongxin, GONG Yu, PENG Hao, WANG Xiaoye. Isolation,Identification and Genome Analysis of Escherichia coli Phage vB_EcoP_GN07 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1522-1531.