猪胸膜肺炎放线杆菌新疆株的分离鉴定及 ApxⅣA基因的克隆与序列分析

Abstract

Abstract: A strain of Actinobacillus pleuropneumoniae（APP） is isolated from 20 cases of swine which may be infected with APP from 4 large-scale pig farms in the north of Xinjiang. The strains were identified by morphologic, cultural and biochemical characteristics. And according to the sequence of APP, the 2427 bp of N-terminal of ApxⅣA was amplified by PCR cloned into pMD19-T vector and sequence analyzed. The results of DNA sequence comparison of the amplified fragment with the published sequence in GenBank showed 98% nucleotide homology, comparing with the three standard strains. The homlogy is separately 98.23%, 99.46%, 98.06%.

Key words: isolation; identification; ApxⅣ cloning; sequencing

CLC Number:

HAO Cheng-wu;WANG Yong-xia;LI Shang-hua;MA Xun. The Isolation and Identifcation of Actinobacillus Pleuropneumoniae and Cloning and Sequence Analysis of ApxIVA Specific Fragment in Xinjiang[J]. , 2010, 37(5): 166-169.

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The Isolation and Identifcation of Actinobacillus Pleuropneumoniae and Cloning and Sequence Analysis of ApxIVA Specific Fragment in Xinjiang

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