奶牛FcRn受体α链基因启动子区的SSCP多态性研究

›› 2010, Vol. 37 ›› Issue (5): 108-111.

• 遗传繁育 • Previous Articles Next Articles

SSCP Polymorphism Research of Promoter of FcRn α Chain Gene in Holstein Dairy Cows

ZHANG Chun-lin1,2, WANG Jia-qi1, ZHAO Sheng-guo1, ZHAO Guo-qi2

(1.College of Animal Science and Technology of Yangzhou University，Yangzhou 225009,China; 2.State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

Received:1900-01-01 Revised:2010-03-09 Online:2010-05-20 Published:2010-05-20
Contact: WANG Jia-qi

Abstract

Abstract: The PCR-SSCP polymorphisms of promoter of Fc receptor (FcRn) α chain (FCGRT) gene were studied in 189 Holstein cows. The results showed that PCR-SSCP polymorphisms were identified in promoter amplified fragment (P1，P2 and P3) of FCGRT gene. For the amplified fragment of P1, the genotype frequency of AA, AB and BB was 25.40%, 59.26% and 15.34%, respectively. For the amplified fragment of P2, the genotype frequency of CC，CD and DD was 20.63%, 56.61% and 22.75%, respectively. For the amplified fragment of P3,the genotype frequency of EE,EF and FF was 1.59%,12.17% and 86.24%, respectively.The sequencing analysis indicated that the polymorphisms were separately due to three single point mutation C to T, C to A and T to A at amplified fragment. Except for SNP1 in Holstein cows,the SNP2 and SNP3 sites in the populations were in a state of Hardy-Weinberg equlibrium.

Key words: Holstein dairy cows; FCGRT promoter; PCR-SSCP

CLC Number:

ZHANG Chun-lin;;WANG Jia-qi;ZHAO Sheng-guo;ZHAO Guo-qi. SSCP Polymorphism Research of Promoter of FcRn α Chain Gene in Holstein Dairy Cows[J]. , 2010, 37(5): 108-111.

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SSCP Polymorphism Research of Promoter of FcRn α Chain Gene in Holstein Dairy Cows

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