猪圆环病毒2型云南株分离鉴定及ORF2序列分析

›› 2010, Vol. 37 ›› Issue (5): 103-107.

• 生物技术 • Previous Articles Next Articles

Isolation and Identification，Squencing and Analyzing of ORF2 Genes of Porcine Circovirus Type 2 from Yunnan Province

LI Peng1,2, FENG Shu-zhang3, TANG Hui-qiong4, LI Wen-gui2, CAI Jun2, LI Zuo-sheng2, ZHANG Yi-fang2

(1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2.College of Animal Science and Technology Yunnan Agricultural University, Kunming 650201, China; 3.Institute of Veterinary Science, the Academy of Military Medical Sciences, Changchun 130062, China; 4.Livestock Station of Wenlai Town, Mengzi County, Yunnan Province, Mengzi 661100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
Contact: ZHANG Yi-fang

Abstract

Abstract: According to the complete genome sequences of porcine circovirus type 2 (PCV2) published in GenBank, two pairs of primers were designed. The ORF2 genomes of three PCV2 isolates were amplified from the samples that were similar to those pigs with PMWS, and approximately 17 nm in diameter under electron microscopy. The results of homology showed that nucleotide homology between the three PCV2 isolates was 99.5%-99.8%, and were closely related to the Zhejiang isolate and the Gansu isolate, sharing 99.7% and 90.7%, and displayed 92.0% and 99.4% nucleotide homology with the Sweden isolate and the South America isolate, respectively.

Key words: porcine circovirus type 2; isolation and identification; sequence analysis

CLC Number:

LI Peng;;FENG Shu-zhang;TANG Hui-qiong;LI Wen-gui;CAI Jun;LI Zuo-sheng;ZHANG Yi-fang. Isolation and Identification，Squencing and Analyzing of ORF2 Genes of Porcine Circovirus Type 2 from Yunnan Province[J]. , 2010, 37(5): 103-107.

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[5]	LIU Xinyue, LI Lifang, WANG Zhenbao, ZHANG Wenliang, ZHANG Xin, ZHAO Hongtao, LI Pengyu, ZHENG Haotian, XIAO Jin, QI Peng. Adjuvants Screening and Immune Protection of Porcine Circovirus Type 2 Synthetic Peptide Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1068-1080.
[6]	ZHOU Wenfeng, ZHANG Yingjie, BAI Yihan, ZHANG Luhua, MAI Jinhui, WANG Naidong, YANG Yi, ZHAN Yang, WANG Dongliang. Complete Genome Sequence Analysis and Cap CTL Epitopes Prediction of Porcine Circovirus Type 2 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 656-665.
[7]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[8]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[9]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[10]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[11]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[12]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[13]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[14]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[15]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.

Isolation and Identification，Squencing and Analyzing of ORF2 Genes of Porcine Circovirus Type 2 from Yunnan Province

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments