绵羊Cdkn1c的DNA甲基化分析

›› 2010, Vol. 37 ›› Issue (4): 85-87.

• 生物技术 • Previous Articles Next Articles

DNA Methylation Pattern of Sheep Cyclin-dependent Kinase Inhibitor 1C

ZHAO Li-xia¹，ZHAO Gao-ping²，GUO Rong-qi³，WANG Bing-ping¹，ZHOU Huan-min^1，2

(1.Bioengineering College，Inner Mongolia Agricultural University，Hohhot 010018，China; 2.College of Animal Science and Veterinary Medicine，Inner Mongolia Agricultural University，Hohhot 010018，China; 3.Hulunbeier Institute of Agricultural Science, Zhalantun 162650，China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20

Abstract

Abstract: In order to determine the DNA methylation profile of sheep Cdkn1c, its mRNA sequences of sheep, cattle, human and mouse were subjected to bioinformatic analysis in the present study. Then bisulfite sequencing method (BSP) was used to examine the DNA methylation pattern of CpG island in Cdkn1c. The results of bioinformatic analysis exhibited that the coding region of Cdkn1c was a big CpG island in four species. And bisulfite sequencing results showed that all the analyzed CpG sites were completely non-methylated in the tissues of kidney and lung, suggesting that the analyzed region was not differential methylated region (DMR) in sheep.

Key words: cyclin-dependent kinase inhibitor 1C; bisulfite sequencing method; DNA methylation; imprinting

CLC Number:

S826

ZHAO Li-xia;ZHAO Gao-ping;GUO Rong-qi;WANG Bing-ping;ZHOU Huan-min;. DNA Methylation Pattern of Sheep Cyclin-dependent Kinase Inhibitor 1C[J]. , 2010, 37(4): 85-87.

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DNA Methylation Pattern of Sheep Cyclin-dependent Kinase Inhibitor 1C

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