›› 2010, Vol. 37 ›› Issue (4): 71-75.

• 生物技术 • Previous Articles     Next Articles

Clone and Sequence Analysis of Dairy Cow’s Mastitis Resistance Gene: Tracheal Antimicrobial Peptide Gene

AO Xue-ping1，LUAN Hong-yu1，YUAN Xi1，CAO Sui-zhong1，2，
YU Shu-min1， LIU Chang-song1， LIU Zong-ping2

  

  1. (1.College of Veterinary Medicine,Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University,Ya’an 625014,China; 2.College of Veterinary Medicine, Yangzhou University,Yangzhou 225009,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: CAO Sui-zhong

Abstract: To investigate characterization of a mastitis resistance gene from dairy cow’s, tracheal antimicrobial peptide (TAP) gene from dairy cow’s mammary gland tissue was amplified by RT-PCR and then cloned into pMD19-T simple vector for sequence analysis. The result of DNA sequencing demonstrated that the cDNA sequence contained a open reading frame (ORF) of 195 bp which shared the highest identity of 93.8% with the TAP of bovine and encoded a 64 amino acid prepro-peptide which contained the β-defensin consensus sequence of six invariantly spaced cysteine residues. Cloning of the complete ORF of TAP may play an important role in the further designing recombinant β-defensins product.

Key words: β-defensins; tracheal antimicrobial peptide(TAP); mammary gland; gene cloning; dairy cow

CLC Number: