›› 2010, Vol. 37 ›› Issue (4): 103-105.

• 生物技术 • Previous Articles     Next Articles

Sequential Analysis of Surface Protein P46 Gene of Mycoplasma hyopneumoniae Wild Strain MY-99

XIONG Ding-jie，XIONG Yan，LIN Lu   

  1. (College of Animal Science and Technology，Sichuan Agricultural University，Ya’an 625014，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: XIONG Yan

Abstract: Mycoplasma hyopneumoniae(Mhp) wild strain MY-99 was cultured in ATCC liquid medium，and extracted DNA. By PCR technique，the specific P46 gene was amplified. Then the P46 gene was cloned into pMD18-T vector and chose two cloned vectors sequenced. The results of sequencing demonstrated that the cloned sequence is 1134 bp，and it is coding 378 amino acids. The sequence is including three TGA codons coding Trp in 182，381 and 734 positions. Comparing the P46 sequence of Mycoplasma hyopneumoniae (Mhp) wild strain MY-99 with the sequence of standard 232 strain published in GenBank, the identity of them were 99.6% and 99.2%.

Key words: Mycoplasma hyopneumoniae (Mhp); P46 gene; sequential analysis

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