Abstract: According to the gene gI and gE clone method，the PCR primer was synthesized, a fragment of 850 bp was carried out, and then PCR product was cloned and sequenced. Then compared others PRV strains, the nucleotide sequence, deduced amino acid sequence homology analysis and the cladogram were made. The result indicated that the nucleotides and amino acids identity of PRV gE gene was 97.0% to 99.3% and 93.2% to 98.5% comparing with other 15 PRV isolates published in GenBank, and with 99.2% nucleotides identity and 98.5% amino acids compared with PRV strain SH. The cladogram indicated that H strain has a close relationship with the PRV strains isolated from domestic other than from abroad. So it can be supposed that the PRV H strain was the epidemic strain in china. 

Key words: pseudorabies virus; gE gene; clone; sequence analysis