基于量子点技术的猪伪狂犬病病毒gB抗体免疫层析试纸研究

doi:10.16431/j.cnki.1671-7236.2023.10.030

Abstract

Abstract: 【Objective】 The objective of this study was to develop a Pseudorabies virus (PRV) gB antibody detection strip based on quantum dots (quantum dots,QDs) technique and provide a rapid and simple detection technique for evaluating the immune efficacy of PRV vaccines. 【Method】 In this study,PRV gB protein expressed by insect cell expression system and carboxyl-modified water-soluble QDs were used,gB-QDs fluorescent coupling compounds were prepared under the action of the coupling agent EDC.Staphylococcus aureus protein A (SPA) and the monoclonal antibodies of anti-gB protein were fixed on the nitrocellulose membrane as detection line and control line,respectively.Sample pads,quantum dots labeled pads,absorbent pads,and support plates were assembled into quantum dots-based fluorescent immunochromatographic strip (QDs-FICS) according to the production process.Then,the sensitivity,specificity of the QDs-FICS and the coincidence rate of it with commercial ELISA kit were detected.【Result】 The sensitivity test results showed that the sensitivity of QDs-FICS was 1∶6 400.The specificity of QDs-FICS showed that the QDs-FICS was highly specific to anti-PRV serum and had no cross-reaction with Classical swine fever virus (CSFV),Foot-and-mouth disease virus (FMDV),Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV) positive serum,the specificity of it was 100%.Through the detection of field pig serum samples and comparing with commercial ELISA kit,the results showed that among the 113 field pig serum samples,11 were inconsistent between the two methods,and 102 were consistent with the results,the coincidence rate of the QDs-FICS with the commercial ELISA kit was 90.3%.【Conclusion】 The color of the test line of the developed strip was clearly visible with high recognition under ultraviolet lamp.The QDs-FICS had a high specificity and sensitivity,it could be used to detect the antibody of PRV gB protein.

Key words: porcine; Pseudorabies virus (PRV); quantum dots; antibody; strip

CLC Number:

S852.4

YANG Suzhen, LIU Yunchao, XING Yunrui, SUN Yaning, SHANG Yanli, ZHANG Gaiping. Study on Quantum Dots-based Fluorescent Immunochromatographic Strip for gB Antibody of Porcine Pseudorabies Virus[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4160-4167.

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Study on Quantum Dots-based Fluorescent Immunochromatographic Strip for gB Antibody of Porcine Pseudorabies Virus

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