›› 2010, Vol. 37 ›› Issue (3): 102-104.

• 生物技术 • Previous Articles     Next Articles

Preliminary Application of Surface Plasmon Resonance (SPR)-Based Biosensor Immunoassay on Detection of Medroxy Progesterone Acetate

HUANG Qiang-li1,2，WANG Jing-yu1，HAN Xue-qing2，LIN Xiang-mei2，WANG Hui-yu2，MEI Lin2   

  1. (1.College of Veterinary Medicine，Northwest A & F University，Yangling 712100， China；2.Chinese Academy of Inspection and Quarantine，Beijing 100029，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: WANG Jing-yu

Abstract: The residues of medroxy progesterone acetate (MPA) in food of animal origin have potential hazards to people's health.A sensitive and rapid immunoassay of a surface plasmon resonance (SPR)-based biosensor was developed for detecting MPA residues, the conditions for immobilizing MPA antigen on the chip were explored, and the reactive concentration of antibody and the conditions for regenerating chip were optimized. The stability and the specificity of the antibody were also evaluated. The results showed that the lowest detective threshold value of SPR-based biosensor immunoassay was about 1 ng/mL MPA. In a parallel assays performed with simulation samples, its detective results were concordance with those of MPA EIA Kit imported. The detective time needed for a single sample was less than 5 min using the SPR-based biosensor.Therefor,it’s very suitable for rapid detection on-site and would be have a broad prospect of application.

Key words: medroxy progesterone acetate; surface plasmon resonance (SPR)-based biosensor; rapid detection

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