China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (2): 59-61.

• 生理生化 • Previous Articles     Next Articles

The Effects on the Activities of Enzymes in Serum by Infusing Staphylococcus aureus via Teat Duct in Rabbits

YAO Xue- ping1, YANG De- ying1, YANG Hong- sen1, WU Jie1, 
YU Shu- min1, CAO Sui- zhong1,2, LIU Zong- ping2
   

  1. (1.Key laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China; 2.College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract: Abstract: In order to screen indicators for earlier diagnosis of diary cow mastitis by investigating the activity dynamic changes of enzymes in serum of rabbits at before and after infusion Staphylococcus aureus through teat canal. Eighteen healthy pregnant rabbits at seven days after parturition were infused with Staphylococcus aureus via teat duct to the fourth pairs of the mammary gland to establish animal model of mastitis. At exactly 2 h before inoculation and 6, 12, 24, 48, 72 h after infusion, taking the blood to determine the activity of alkaline phosphatase (ALP), lactatedehydrogenase (LDH), lactoperoxidase (LP) and myeloperoxidase (MPO). At different time after infusion, one rabbit was euthanatized and the mammary glands were removed and fixed for histopathologic evaluations. The results showed that the activity of ALP, LDH and MPO significantly increased within 6-48 h (P<0.05), and activity of LP significantly increased (P<0.05) within 12-48 h after infusion Staphylococcus aureus via teat canal. These four enzymes activities reached peak value at 12 h after infusion. The fitting degree (R2=0.9074) between LDH activity with different time at before and after infusion Staphylococcus aureus higher than other three enzymes. Mammary tissue damage was closely related to the LDH activity. The results suggested that LDH could be as a candidate indicator for earlier mastitis diagnosis to further study.

Key words: Key words: rabbit; mastitis; Staphylococcus aureus; serum enzymes; animal model of mastitis