猪繁殖与呼吸综合征病毒河南分离株GP4蛋白免疫活性研究

›› 2010, Vol. 37 ›› Issue (1): 57-60.

• 生物技术 • Previous Articles Next Articles

Immunologic Competence of the GP4 Protein of Porcine Reproductive and Respiratory Syndrome Virus Henan Strain

ZHAO Lin, WANG Zhong-ming, XIA Ping-an, ZHANG Feng-hua, LIU Yu-song, FAN Xu, XU Hong-yun, CUI Bao-an

(College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: XIA Ping-an

Abstract

Abstract: ORF4 gene of PRRSV Henan strain was cloned. By deletion hydrophobic sequence and cloned into the prokaryotic expression vector pET-32a, positive recombinant was successfully obtained with the induction of IPTG. Analyze purificated protein by western blotting. Immunifaction thought cytorrhyctes, degenerated protein, reannealing protein. Detect titer and neutralization of the blood serum. It can be used for the further investigation on immunogenicity of GP4 protein of PRRSV.

Key words: PRRSV; GP4 protein; immunologic competence

CLC Number:

ZHAO Lin;WANG Zhong-ming;XIA Ping-an;ZHANG Feng-hua;LIU Yu-song;FAN Xu;XU Hong-yun;CUI Bao-an. Immunologic Competence of the GP4 Protein of Porcine Reproductive and Respiratory Syndrome Virus Henan Strain[J]. , 2010, 37(1): 57-60.

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Immunologic Competence of the GP4 Protein of Porcine Reproductive and Respiratory Syndrome Virus Henan Strain

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