从全长cDNA克隆恢复猪瘟病毒方法的研究

›› 2009, Vol. 36 ›› Issue (6): 56-60.

• 生物技术 • Previous Articles Next Articles

The Study of Recovered Infectious Classical Swine Fever Virus from Full-length cDNA Clone

FAN Yun-feng^1,2，ZHAO Qi-zu¹, ZHAO Yun¹, ZOU Xing-qi¹, ZHANG Zhong-qiu^1,2，WANG Qin¹, NING Yi-bao¹

(1.China Institute of Veterinary Drug Control, Beijing 100081, China; 2.China Animal Disease Control Center, Beijing 100026, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20

Abstract

Abstract: The viral RNA was synthesized in vitro with T7 RNA polymerase using recombinant plasmid pAC/F101/T1-7 as a model, which contained the complete genome of CSFV Thiverval strain, and transfected into PK-15 and BHK-21 cells. After the cell passage, RT-PCR detection and immunoperoxidase monolayer assay with E2 monoclonal antibody, the results indicated that the virus had been rescued successfully from the Thiverval strain full-length cDNA clone in two cells. At the same time, through the comparison of transfected efficiency of PK-15 and BHK-21 cells, the new method of CSFV virus rescue was established that using the high thansfected efficiency cells as transfection and porcine kidney cells as multiplication. This method highly enhanced efficiency of CSFV virus rescue.

Key words: classical swine fever virus; Thiverval strain; full-length cDNA clone; virus rescue

CLC Number:

S852.65⁺9.6

FAN Yun-feng;ZHAO Qi-zu;ZHAO Yun;ZOU Xing-qi;ZHANG Zhong-qiu;WANG Qin;NING Yi-bao. The Study of Recovered Infectious Classical Swine Fever Virus from Full-length cDNA Clone[J]. , 2009, 36(6): 56-60.

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The Study of Recovered Infectious Classical Swine Fever Virus from Full-length cDNA Clone

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