广西猪瘟病毒E0和E2基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.04.003

Abstract

Abstract: In order to provide the scientific basis for molecular epidemiology, genetic variation, comprehensive prevention and control of classical swine fever in Guangxi, the molecular characteristic of E0 and E2 genes of classical swine fever virus (CSFV) in Guangxi was analyzed. The E0 and E2 genes of CSFV from positive samples of swine was amplified using RT-PCR, cloned into the pMD18-T vector and sequenced. The sequences were analyzed using DNAStar software and the phylogenetic trees were drawn. The result indicated that the E0 and E2 genes of CSFV were amplified from positive samples, the homologies of nucleotide sequence and amino acid sequences of the GX2 strain to E0 gene of reference strains were 83.1% to 94 1% and 85.9% to 99.6%, respectively. The homologies of nucleotide sequence and amino acid sequences of the GX2 strain to E2 gene of reference strains were 81.7% to 93.7% and 89.0% to 97.0%. The E0 and E2 genes of CSFV belonged to group Ⅱ. No variation occurred at E0 protein amino acid sequences of two domains of RNase activity. No change was observed in 15 Cys sites of E2 protein of the GX2 strain. However, the variation of amino acids at sites S734R was observed. The variation trend of GX2 strain was similar to CSFV strains which were isolated in recent years in Guangxi. GX2 strain shared low homology with HCLV and Shimen strains and was not close related to HCLV and Shimen, but shared high homology with GXWZ02.

Key words: classical swine fever virus; E0 gene; E2 gene; cloning; sequences analysis

CLC Number:

Q785

CHEN Tian-jiao, HE Qi-song, YAN Jian-hua, XIONG Yi, FENG Shu-ping, LAN Jia-nuan, LIAO Ling-ling. Cloning and Sequencing of Classical Swine Fever Virus E0 and E2 Genes in Guangxi[J]. , 2015, 42(4): 789-795.

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Cloning and Sequencing of Classical Swine Fever Virus E0 and E2 Genes in Guangxi

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